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Literature summary extracted from

  • Chung, J.S.; Noguera-Mazon, V.; Lancelin, J.M.; Kim, S.K.; Hirasawa, M.; Hologne, M.; Leustek, T.; Knaff, D.B.
    Interaction domain on thioredoxin for Pseudomonas aeruginosa 5'-adenylylsulfate reductase (2009), J. Biol. Chem., 284, 31181-31189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.8
-
 Pseudomonas aeruginosa

General Stability

EC Number General Stability Organism
1.8.4.10 the enzyme is unstable at 30°C in 20 mM phosphate buffer (pH 5.7) Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.4.8 0.01
-
 thioredoxin I wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4 Pseudomonas aeruginosa
1.8.4.10 0.01
-
 thioredoxin wild type enzyme, at 20°C in 10 mM phosphate buffer (pH 7.5) containing 100 mM Na2SO4 Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.8 Pseudomonas aeruginosa
-
-
-
1.8.4.10 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.8
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.8 adenosine 5'-phosphosulfate + thioredoxin I
-
 Pseudomonas aeruginosa AMP + sulfite + oxidized thioredoxin I
-
 ?
1.8.4.8 additional information thioredoxin I mutant W31A shows no detectable activity, whereas W31F, K36E, and D61N are able to serve as electron donors for the APR-catalyzed reaction but with lower turnover numbers than that exhibited by the wild type thioredoxin I. The Km for thioredoxin mutant R73E is increased by 7.7fold compared with wild type thioredoxin I Pseudomonas aeruginosa ?
-
 ?
1.8.4.10 adenylyl sulfate + thioredoxin
-
 Pseudomonas aeruginosa AMP + sulfite + thioredoxin disulfide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.8 5'-adenylylsulfate reductase
-
 Pseudomonas aeruginosa
1.8.4.8 APR
-
 Pseudomonas aeruginosa
1.8.4.8 APS reductase
-
 Pseudomonas aeruginosa
1.8.4.10 5'-adenylylsulfate reductase
-
 Pseudomonas aeruginosa
1.8.4.10 APR
-
 Pseudomonas aeruginosa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.8.4.10 5.7
-
 the enzyme is unstable at 30°C in 20 mM phosphate buffer (pH 5.7) Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.8 thioredoxin dependent on Pseudomonas aeruginosa
1.8.4.10 thioredoxin
-
 Pseudomonas aeruginosa