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Literature summary extracted from

  • Menon, B.R.; Waltho, J.P.; Scrutton, N.S.; Heyes, D.J.
    Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase (2009), J. Biol. Chem., 284, 18160-18166.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.33 expression in Escherichia coli cells Thermosynechococcus vestitus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.33 K197A mutant, constructed for analysing the role of the conserved active site lysine Thermosynechococcus vestitus
1.3.1.33 K197Q mutant, constructed for analysing the role of the conserved active site lysine Thermosynechococcus vestitus
1.3.1.33 K197R mutant, constructed for analysing the role of the conserved active site lysine Thermosynechococcus vestitus
1.3.1.33 Y193A mutant, constructed for analysing the role of the conserved active site tyrosine Thermosynechococcus vestitus
1.3.1.33 Y193F mutant, constructed for analysing the role of the conserved active site tyrosine Thermosynechococcus vestitus
1.3.1.33 Y193S mutant, constructed for analysing the role of the conserved active site tyrosine Thermosynechococcus vestitus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.33 additional information
-
 protochlorophyllide mutant K197A, relative activity 2.7% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant K197Q, relative activity 2.0% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant K197R, relative activity 2.3% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant Y193A, relative activity 5.5% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant Y193F, relative activity 19.5% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide mutant Y193S, relative activity 2.4% Thermosynechococcus vestitus
1.3.1.33 additional information
-
 protochlorophyllide wild type enzyme, relative activity 100% Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+ Thermosynechococcus vestitus activation by light chlorophyllide + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.33 Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.33
-
 Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.33 protochlorophyllide + NADPH + H+ activation by light Thermosynechococcus vestitus chlorophyllide + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.33 POR
-
 Thermosynechococcus vestitus
1.3.1.33 protochlorophyllide oxidoreductase
-
 Thermosynechococcus vestitus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.33 25
-
 activity assay Thermosynechococcus vestitus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.33 0.003
-
 protochlorophyllide mutant K197Q Thermosynechococcus vestitus
1.3.1.33 0.004
-
 protochlorophyllide mutant K197A Thermosynechococcus vestitus
1.3.1.33 0.004
-
 protochlorophyllide mutant K197R Thermosynechococcus vestitus
1.3.1.33 0.004
-
 protochlorophyllide mutant Y193S Thermosynechococcus vestitus
1.3.1.33 0.009
-
 protochlorophyllide mutant Y193A Thermosynechococcus vestitus
1.3.1.33 0.032
-
 protochlorophyllide mutant Y193F Thermosynechococcus vestitus
1.3.1.33 0.164
-
 protochlorophyllide wild type enzyme Thermosynechococcus vestitus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.33 7.5
-
 activity assay Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.33 NADPH
-
 Thermosynechococcus vestitus