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Literature summary extracted from

  • Saito, N.; Robert, M.; Kochi, H.; Matsuo, G.; Kakazu, Y.; Soga, T.; Tomita, M.
    Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in Escherichia coli (2009), J. Biol. Chem., 284, 16442-16451.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.61 expressed as a histidine-tagged recombinant protein in Escherichia coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.61 1
-
 3-hydroxypropane sulfonate
-
 Escherichia coli
1.1.1.61 1
-
 3-hydroxypropane sulfonate in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli
1.1.1.61 4.3
-
 Succinic semialdehyde
-
 Escherichia coli
1.1.1.61 4.3
-
 Succinic semialdehyde in 100 mM MOPS-KOH buffer (pH 7.2), at 37°C Escherichia coli
1.1.1.61 9
-
 methylglyoxal
-
 Escherichia coli
1.1.1.61 9
-
 methylglyoxal in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli
1.1.1.61 102
-
 4-hydroxybutyrate
-
 Escherichia coli
1.1.1.61 102
-
 gamma-hydroxybutyrate in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.61 additional information no significant differences in the activity are observed when using different divalent metals (Mg2+, Mn2+, Ca2+, Co2+, and Zn2+) Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.61 31154
-
 4 * 31154, calculated from amino acid sequence Escherichia coli
1.1.1.61 143000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.61 gamma-hydroxybutyrate + NAD+ Escherichia coli
-
 succinic semialdehyde + NADH + H+
-
 r
1.1.1.61 succinic semialdehyde + NADH + H+ Escherichia coli succinic semialdehyde is the preferred substrate gamma-hydroxybutyrate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.61 Escherichia coli
-
-
-
1.1.1.61 Escherichia coli P0A9V8
-
-
1.1.1.61 Escherichia coli K12 BW25113 P0A9V8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.61 purified using cobalt-based immobilized TALON metal affinity chromatography resins with a gravity-flow column Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.61 0.062
-
 substrate: gamma-hydroxybutyrate Escherichia coli
1.1.1.61 0.067
-
 substrate: methylglyoxal Escherichia coli
1.1.1.61 0.2
-
 substrate: succinic semialdehyde Escherichia coli
1.1.1.61 9
-
 substrate: 3-hydroxypropane sulfonate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.61 3-hydroxypropane sulfonate + NAD+
-
 Escherichia coli ? + NADH
-
 ?
1.1.1.61 3-hydroxypropane sulfonate + NAD+
-
 Escherichia coli K12 BW25113 ? + NADH
-
 ?
1.1.1.61 3-hydroxypropane sulfonate + NAD+
-
 Escherichia coli ? + NADH + H+
-
 r
1.1.1.61 gamma-hydroxybutyrate + NAD+
-
 Escherichia coli succinic semialdehyde + NADH + H+
-
 r
1.1.1.61 methylglyoxal + NAD+
-
 Escherichia coli ? + NADH
-
 ?
1.1.1.61 methylglyoxal + NAD+
-
 Escherichia coli K12 BW25113 ? + NADH
-
 ?
1.1.1.61 methylglyoxal + NAD+
-
 Escherichia coli ? + NADH + H+
-
 r
1.1.1.61 additional information no activity with D-glycerate and 3-hydroxypropanoate Escherichia coli ?
-
 ?
1.1.1.61 succinic semialdehyde + NADH + H+
-
 Escherichia coli 4-hydroxybutyrate + NAD+
-
 r
1.1.1.61 succinic semialdehyde + NADH + H+
-
 Escherichia coli K12 BW25113 4-hydroxybutyrate + NAD+
-
 r
1.1.1.61 succinic semialdehyde + NADH + H+ succinic semialdehyde is the preferred substrate Escherichia coli gamma-hydroxybutyrate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.61 homotetramer 4 * 31154, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.1.1.61 gamma-hydroxybutyrate dehydrogenase
-
 Escherichia coli
1.1.1.61 GHBDH
-
 Escherichia coli
1.1.1.61 uncharacterized oxidoreductase YihU
-
 Escherichia coli
1.1.1.61 yihU
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.61 NAD+
-
 Escherichia coli
1.1.1.61 NADH
-
 Escherichia coli
1.1.1.61 NADH the NADH-dependent succinic semialdehyde reductase activity is 4fold higher than that with NADPH Escherichia coli

General Information

EC Number General Information Comment Organism
1.1.1.61 malfunction a yihU deletion mutant displays reduced tolerance to the cytotoxic effects of exogenous addition of succinic semialdehyde Escherichia coli
1.1.1.61 metabolism the enzyme is involved in the metabolism of succinic semialdehyde, and other potentially toxic intermediates that may accumulate under stress conditions in Escherichia coli Escherichia coli