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Literature summary extracted from

  • Yu, W.; Lin, Y.; Yao, J.; Huang, W.; Lei, Q.; Xiong, Y.; Zhao, S.; Guan, K.L.
    Lysine 88 acetylation negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals (2009), J. Biol. Chem., 284, 13669-13675.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.1.3.3 medicine ornithine carbamoyltransferase is acetylated at lysine resiudes, including K88, which is also mutated in ornithine carbamoyltransferase-deficient patients. K88 acetylation decreases the affinity for carbamoyl phosphate, and the maximum velocity, whereas the Km for ornithine is not affected Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.3.3 K88Q less than 1% of wild-type activity Homo sapiens
2.1.3.3 K88R mutant retains substantial enzymatic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.3 0.13
-
Carbamoyl phosphate wild-type, pH 7.7, 37°C Homo sapiens
2.1.3.3 0.15
-
Carbamoyl phosphate mutant K88R, pH 7.7, 37°C Homo sapiens
2.1.3.3 0.36
-
L-ornithine wild-type, pH 7.7, 37°C Homo sapiens
2.1.3.3 0.42
-
L-ornithine mutant K88R, pH 7.7, 37°C Homo sapiens
2.1.3.3 0.55
-
L-ornithine mutant K88Q, pH 7.7, 37°C Homo sapiens
2.1.3.3 1.24
-
Carbamoyl phosphate mutant K88Q, pH 7.7, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.1.3.3 additional information ornithine carbamoyltransferase is acetylated at lysine residues, including K88, which is also mutated in ornithine carbamoyltransferase-deficient patients. K88 acetylation decreases the affinity for carbamoyl phosphate, and the maximum velocity, whereas the Km for ornithine is not affected. K88 acetylation is regulated by both extracellular glucose and amino acid availabilit Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.3.3 Chang liver cell
-
Homo sapiens
-
2.1.3.3 HEK-293T cell
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
Homo sapiens phosphate + L-citrulline
-
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