Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Claperon, C.; Banegas-Font, I.; Iturrioz, X.; Rozenfeld, R.; Maigret, B.; Llorens-Cortes, C.
    Identification of threonine 348 as a residue involved in aminopeptidase A substrate specificity (2009), J. Biol. Chem., 284, 10618-10626.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.7 wild type and mutant enzymes are expressed in CHO-K1 cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.7 T348D the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ Mus musculus
3.4.11.7 T348S mutant with increased activity, the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ Mus musculus
3.4.11.7 T348Y mutant with strongly reduced activity, the mutation leads to a modification of the hydrolysis velocity, with no change in the affinity of the recombinant enzymes for the substrate L-Glu-2-naphthylamide, either in the absence or presence of Ca2+ Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.7 glutamate phosphonate
-
Mus musculus
3.4.11.7 glutamate thiol
-
Mus musculus
3.4.11.7 lysine thiol
-
Mus musculus
3.4.11.7 methionine thiol
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.7 0.159
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.198
-
L-Glu-2-naphthylamide recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.258
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.282
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 1.136
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 1.481
-
L-Glu-2-naphthylamide recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 1.919
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 2.034
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.7 membrane
-
Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.7 Ca2+ the presence of 4 mM Ca2+ enhances the enzymatic activity of wild type enzyme by a factor of 8 Mus musculus
3.4.11.7 Zn2+ dependent on Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.7 140000
-
minor immature form, SDS-PAGE Mus musculus
3.4.11.7 160000
-
major mature form, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.7 Mus musculus P16406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.7 Talon Co2+ column chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.7 brain
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.7 0.25
-
mutant enzyme T348Y, in the absence of Ca2+ Mus musculus
3.4.11.7 0.8
-
mutant enzyme T348D, in the absence of Ca2+ Mus musculus
3.4.11.7 1.6
-
mutant enzyme T348D, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 1.98
-
wild type enzyme, in the absence of Ca2+ Mus musculus
3.4.11.7 3.05
-
mutant enzyme T348S, in the absence of Ca2+ Mus musculus
3.4.11.7 7.4
-
mutant enzyme T348Y, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 14.67
-
wild type enzyme, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 31.54
-
mutant enzyme T348S, in the presence of 4 mM Ca2+ Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.7 angiotensin II + H2O
-
Mus musculus aspartic acid + angiotensin III
-
?
3.4.11.7 L-Glu-2-naphthylamide + H2O
-
Mus musculus L-Glu + 2-naphthylamine
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.7 aminopeptidase A
-
Mus musculus
3.4.11.7 APA
-
Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.7 2 8 L-Glu-2-naphthylamide recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 40
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 92
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 167
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 256
-
L-Glu-2-naphthylamide recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 292
-
L-Glu-2-naphthylamide recombinant wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 575
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 640
-
L-Glu-2-naphthylamide recombinant mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.7 0.0001
-
lysine thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00019
-
methionine thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00022
-
lysine thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00026
-
methionine thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00026
-
lysine thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00029
-
methionine thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00038
-
glutamate thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00058
-
methionine thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00085
-
glutamate thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.0009
-
lysine thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00103
-
glutamate thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.0011
-
glutamate thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00194
-
glutamate thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00197
-
glutamate thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.002
-
glutamate thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00206
-
glutamate thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the absence of Ca2+ Mus musculus
3.4.11.7 0.00275
-
lysine thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00298
-
lysine thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00307
-
methionine thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.0036
-
lysine thiol mutant enzyme T348Y, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00394
-
lysine thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00403
-
methionine thiol mutant enzyme T348S, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.00667
-
methionine thiol mutant enzyme T348D, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus
3.4.11.7 0.0084
-
methionine thiol wild type enzyme, in 50 mM Tris-HCl buffer, pH 7.4, in the presence of 4 mM Ca2+ Mus musculus