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Literature summary extracted from

  • Bowling, K.M.; Huang, Z.; Xu, D.; Ferdousy, F.; Funderburk, C.D.; Karnik, N.; Neckameyer, W.; O'Donnell, J.M.
    Direct binding of GTP cyclohydrolase and tyrosine hydroxylase: regulatory interactions between key enzymes in dopamine biosynthesis (2008), J. Biol. Chem., 283, 31449-31459.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.16.2 GTP cyclohydrolase I as concentrations of 5,6,7,8-tetrahydrobiopterin are increased (0.025 and 0.1 mM), the specific activity of tyrosine hydroxylase is stimulated in the presence of GTP cyclohydrolase I isoform C Drosophila melanogaster
3.5.4.16 additional information GTP cyclohydrolase I is activated by phosphorylation with protein kinase C or A Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.16.2 expressed in Escherichia coli BL21(DE3)pLysS cells Drosophila melanogaster
3.5.4.16 expressed in Escherichia coli BL21(DE3)pLysS cells Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.16.2 dopamine feedback inhibition, in the presence of 0.02 mM dopamine tyrosine hydroxylase enzyme specific activity is reduced by about 60% Drosophila melanogaster
3.5.4.16 tetrahydrobiopterin
-
 Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.16.2 additional information Drosophila melanogaster tyrosine hydroxylase stimulates GTP cyclohydrolase I activity ?
-
 ?
3.5.4.16 additional information Drosophila melanogaster GTP cyclohydrolase I stimulates tyrosine hydroxylase activity by increasing the maximal velocity of the enzyme and fails to block the feedback inhibition of tyrosine hydroxylase by dopamine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.16.2 Drosophila melanogaster
-
-
-
3.5.4.16 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.16.2 ammonium sulfate precipitation and Sephacryl S-300HR gel filtration Drosophila melanogaster
3.5.4.16 ammonium sulfate precipitation, Ni-NTA column chromatography, and Sephacryl S-300HR gel filtration Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.16.2 head
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.16.2 L-tyrosine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Drosophila melanogaster 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin
-
 ?
1.14.16.2 additional information tyrosine hydroxylase stimulates GTP cyclohydrolase I activity Drosophila melanogaster ?
-
 ?
3.5.4.16 GTP + H2O
-
 Drosophila melanogaster formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
3.5.4.16 additional information GTP cyclohydrolase I stimulates tyrosine hydroxylase activity by increasing the maximal velocity of the enzyme and fails to block the feedback inhibition of tyrosine hydroxylase by dopamine Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.16.2 tyrosine hydroxylase
-
 Drosophila melanogaster
3.5.4.16 GTP cyclohydrolase I
-
 Drosophila melanogaster
3.5.4.16 GTPCH
-
 Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.16.2 tetrahydrobiopterin
-
 Drosophila melanogaster