EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.43 | Triton X-100 | transferasetransfer of the 4-amino-4-deoxy-alpha-L-arabinose unit from its putative isoprenoid carrier, undecaprenyl phosphate-4-amino-4-deoxy-alpha-L-arabinose, to the radiolabeled acceptor [4'-32P]lipid IV(A) is dependent upon the presence of the nonionic detergent Triton X-100, with maximal activity observed at 0.2% in the assay system | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.B4 | overxpression of the L-Ara4N lipid A transferase (ArnT) behind a T7lac promoter | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | overxpression of the L-Ara4N lipid A transferase (ArnT) behind a T7lac promoter | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.43 | Ca2+ | Cacl2, inhibits above 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.4.2.43 | Mg2+ | Mgcl2, inhibits above 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.2.B4 | membrane | inner membrane | Salmonella enterica subsp. enterica serovar Typhimurium | 16020 | - |
2.4.2.43 | membrane | inner membrane | Salmonella enterica subsp. enterica serovar Typhimurium | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.B4 | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | attachment of the cationic sugar 4-amino-4-deoxy-L-arabinose to lipid A is required for the maintenance of polymyxin resistance in Salmonella typhimurium. Lipid A molecules containing a Kdo disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Transfer to lipid A occurs on the periplasmic side of the inner membrane | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate | - |
? | |
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate + lipid IV(A) | Salmonella enterica subsp. enterica serovar Typhimurium | ArnT adds a single 4-amino-4-deoxy-alpha-L-arabinose unit to the 1-phosphate moiety of the tetraacylated lipid A precursor, lipid IV(A), which lacks Kdo and forms lipid II (A). Transfer to lipid A occurs on the periplasmic side of the inner membrane. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Lipid IV(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose. Lipid II(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-alpha-D-glucopyranose | lipid II(A) + undecaprenyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.B4 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
2.4.2.43 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.B4 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA = lipid IIA + ditrans,octacis-undecaprenyl phosphate | lipid IV(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose. Lipid II(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-alpha-D-glucopyranose | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.B4 | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate | attachment of the cationic sugar 4-amino-4-deoxy-L-arabinose to lipid A is required for the maintenance of polymyxin resistance in Salmonella typhimurium. Lipid A molecules containing a Kdo disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Transfer to lipid A occurs on the periplasmic side of the inner membrane | Salmonella enterica subsp. enterica serovar Typhimurium | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate | - |
? | |
2.4.2.B4 | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate | lipid A molecules containing a KDO disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent | Salmonella enterica subsp. enterica serovar Typhimurium | 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate | - |
? | |
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate + lipid IV(A) | ArnT adds a single 4-amino-4-deoxy-alpha-L-arabinose unit to the 1-phosphate moiety of the tetraacylated lipid A precursor, lipid IV(A), which lacks Kdo and forms lipid II (A). Transfer to lipid A occurs on the periplasmic side of the inner membrane. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Lipid IV(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose. Lipid II(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-alpha-D-glucopyranose | Salmonella enterica subsp. enterica serovar Typhimurium | lipid II(A) + undecaprenyl phosphate | - |
? | |
2.4.2.43 | 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate + lipid IV(A) | ArnT adds a single 4-amino-4-deoxy-alpha-L-arabinose unit to the 1-phosphate moiety of the tetraacylated lipid A precursor. Lipid IV(A), which lacks Kdo and forms lipid II (A)lipid IV(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose. Lipid II(A) = 2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-alpha-D-glucopyranose | Salmonella enterica subsp. enterica serovar Typhimurium | lipid II(A) + undecaprenyl phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.B4 | L-Ara4N transferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | L-Ara4N transferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.B4 | 30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | 30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.B4 | 6.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
2.4.2.43 | 6.5 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |