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Literature summary extracted from

  • Potter, D.; Miziorko, H.M.
    Identification of catalytic residues in human mevalonate kinase (1997), J. Biol. Chem., 272, 25449-25454.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.36 D204A stable, with 40000fold diminution in kcat. Mutant is able to bind a spin-labeled ATP analogue with stoichiometries and equilibrium binding constants comparable to wild-type Homo sapiens
2.7.1.36 D204N stable, with 40000fold diminution in kcat. Mutant is able to bind a spin-labeled ATP analogue with stoichiometries and equilibrium binding constants comparable to wild-type Homo sapiens
2.7.1.36 E193A labile Homo sapiens
2.7.1.36 E193Q 50fold diminution in Vmax and 20fold increase Km values for ATP, 40fold increase in Km for mevalonate Homo sapiens
2.7.1.36 E19A destabilization Homo sapiens
2.7.1.36 E19D stable, decrease in Vmax to 40% of wild-type Homo sapiens
2.7.1.36 E19Q destabilization Homo sapiens
2.7.1.36 E296Q stable. Kinetic parameters similar to wild-type Homo sapiens
2.7.1.36 H20A insoluble upon expression in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.36 0.012
-
 mevalonate mutant D204N, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.024
-
 mevalonate wild-type, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.025
-
 ATP mutant D204A, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.052
-
 mevalonate mutant E19D, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.066
-
 ATP mutant D204N, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.074
-
 ATP wild-type, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.11
-
 mevalonate mutant E296Q, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.113
-
 ATP mutant E19D, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.131
-
 ATP mutant E296Q, pH 7.0, 30°C Homo sapiens
2.7.1.36 0.2
-
 mevalonate mutant D204A, pH 7.0, 30°C Homo sapiens
2.7.1.36 1.02
-
 mevalonate mutant E193Q, pH 7.0, 30°C Homo sapiens
2.7.1.36 1.56
-
 ATP mutant E193Q, pH 7.0, 30°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 42000
-
 2 * 42000, SDS-PAGE Homo sapiens
2.7.1.36 78000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 recombinant enzyme Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.36 ATP + (R)-mevalonate = ADP + (R)-5-phosphomevalonate role for residue E193 in interacting with the cation of the MgATP substrate, and for D204 as the catalytic base that facilitates deprotonation of the C5 hydroxyl of mevalonic acid Homo sapiens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 37
-
 pH 7.0, 30°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + mevalonate
-
 Homo sapiens ADP + phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 dimer 2 * 42000, SDS-PAGE Homo sapiens