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Literature summary extracted from

  • Schulz, H.
    Long chain enoyl coenzyme A hydratase from pig heart (1974), J. Biol. Chem., 249, 2704-2709.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.74 iodoacetamide 5 mM, 16% inhibition Sus scrofa
4.2.1.74 N-Methylmaleimide 1 mM, 69% inhibition, substrate: trans-octenoyl-CoA Sus scrofa
4.2.1.74 p-chloromercuribenzoate 1 mM, complete inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.74 0.024
-
 trans-2-decenoyl-CoA 25°C Sus scrofa
4.2.1.74 0.024
-
 trans-2-dodecenoyl-CoA 25°C Sus scrofa
4.2.1.74 0.024
-
 trans-2-octenoyl-CoA 25°C Sus scrofa
4.2.1.74 0.024
-
 trans-2-tetradecenoyl-CoA 25°C Sus scrofa
4.2.1.74 0.045
-
 trans-2-hexenoyl-CoA 25°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.74 mitochondrion
-
 Sus scrofa 5739
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.74 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.74 partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.74 heart
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.74 - 20°C, stable for several months Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.74 additional information activity with crotonyl-CoA is 1% of the activity with trans-decenoyl-CoA Sus scrofa ?
-
 ?
4.2.1.74 trans-2-decenoyl-CoA + H2O Vmax is 74% of Vmax for trans-octenoyl-CoA Sus scrofa (3S)-3-hydroxydecanoyl-CoA
-
 ?
4.2.1.74 trans-2-dodecenoyl-CoA + H2O Vmax is 42% of Vmax for trans-octenoyl-CoA Sus scrofa (3S)-3-hydroxydodecanoyl-CoA
-
 ?
4.2.1.74 trans-2-hexenoyl-CoA + H2O Vmax is 78% of Vmax for trans-octenoyl-CoA Sus scrofa (3S)-3-hydroxyhexanoyl-CoA
-
 ?
4.2.1.74 trans-2-octenoyl-CoA + H2O best substrate Sus scrofa (3S)-3-hydroxyoctanoyl-CoA
-
 ?
4.2.1.74 trans-2-tetradecenoyl-CoA + H2O Vmax is 34% of Vmax for trans-octenoyl-CoA Sus scrofa (3S)-3-hydroxytetradecanoyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.74 long chain enoyl coenzyme A hydratase
-
 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.74 8.5
-
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.74 7 9 pH 7: about 50% of maximal activity, pH 9: 80% of maximal activity Sus scrofa