Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fujisawa, D.; Yamazaki, Y.; Morita, T.
    Re-evaluation of M-LAO, L-amino acid oxidase, from the venom of Gloydius blomhoffi as an anticoagulant protein (2009), J. Biochem., 146, 43-49.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 m-chlorobenzoate LAO inhibitor, does not inhibit the degradation of fibrinogen Gloydius blomhoffii
1.4.3.2 o-aminobenzoic acid LAO inhibitor, slightly inhibiting the degradation of fibrinogen Gloydius blomhoffii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 60000
-
 x * 60000, SDS-PAGE Gloydius blomhoffii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 additional information Gloydius blomhoffii the LAO-containing fraction inhibits coagulation factor IX, and it prolongs the APTT, PT and fibrinogen clotting time and cleaves the Aalpha-chain of fibrinogen probably due to the presence of traces of a metalloproteinase in the fraction, overview. The mechanism for the anticoagulant activity of M-LAO fraction is not due to binding to factor IX. The fibrinogenolytic activity of the M-LAO fraction is independent of either LAO enzymatic activity or generated hydrogen peroxide ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Gloydius blomhoffii Q90W54
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 flavoprotein
-
 Gloydius blomhoffii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 native enzyme by gel filtration, cation and anion exchange chromatography, and hydroxyapatite chromatography Gloydius blomhoffii

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 DL-norleucine + H2O + O2
-
 Gloydius blomhoffii 2-oxocaproate + NH3 + H2O2
-
 ?
1.4.3.2 additional information the LAO-containing fraction inhibits coagulation factor IX, and it prolongs the APTT, PT and fibrinogen clotting time and cleaves the Aalpha-chain of fibrinogen probably due to the presence of traces of a metalloproteinase in the fraction, overview. The mechanism for the anticoagulant activity of M-LAO fraction is not due to binding to factor IX. The fibrinogenolytic activity of the M-LAO fraction is independent of either LAO enzymatic activity or generated hydrogen peroxide Gloydius blomhoffii ?
-
 ?
1.4.3.2 additional information L-amino acid oxidase is a dimeric glycosylated flavoenzyme that catalyses the oxidative deamination of an L-amino acid substrate to a 2-oxo acid along with the production of ammonia and hydrogen peroxide Gloydius blomhoffii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 ? x * 60000, SDS-PAGE Gloydius blomhoffii

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 M-LAO
-
 Gloydius blomhoffii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 37
-
 assay at Gloydius blomhoffii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7.4
-
 assay at Gloydius blomhoffii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 flavin LAO is a flavoenzyme Gloydius blomhoffii