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Literature summary extracted from

  • Kawahara, K.; Mogi, T.; Tanaka, T.Q.; Hata, M.; Miyoshi, H.; Kita, K.
    Mitochondrial dehydrogenases in the aerobic respiratory chain of the rodent malaria parasite Plasmodium yoelii yoelii (2009), J. Biochem., 145, 229-237.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 5-Hydroxy-2-methyl-1,4-naphthoquinone inhibition is only 50% even at 0.1 mM Plasmodium yoelii yoelii
1.3.5.1 atpenin A5
-
 Plasmodium yoelii yoelii
1.3.5.1 carboxin
-
 Plasmodium yoelii yoelii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.00017
-
 ubiquinone-2 differences in Km value (9fold) and Vmax/Km ratio (19fold) between Q1 and Q2 indicate that the 6-polyprenyl tail of the ubiquinone ring contributes to the binding affinity and that Q2 is better substrate than Q1 Plasmodium yoelii yoelii
1.3.5.1 0.0016
-
 ubiquinone-1
-
 Plasmodium yoelii yoelii
1.3.5.1 0.049
-
 succinate
-
 Plasmodium yoelii yoelii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 mitochondrion enzyme isolated from mitochondria Plasmodium yoelii yoelii 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 30000
-
 sum of molecular weights of membrane anchor subunits is about 30 kDa Plasmodium yoelii yoelii
1.3.5.1 35000
-
 iron-sulphur subunit Plasmodium yoelii yoelii
1.3.5.1 70000
-
 flavoprotein subunit, identified by Western blot analysis Plasmodium yoelii yoelii
1.3.5.1 135000
-
 high resolution clear-native electrophoresis Plasmodium yoelii yoelii

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Plasmodium yoelii yoelii
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.1 additional information
-
 when comparing with rat liver mitochondria, succinate-quinone reductase activity of Plasmodium yoelii yoelii, is 1.4% of rat liver succinate-quinone reductase Plasmodium yoelii yoelii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 succinate + ubiquinone-1 succinate:quinone reductase activity is determined as quinone-mediated succinate:2,4-dichlorophenolindophenol (DCIP) reductase Plasmodium yoelii yoelii fumarate + ubiquinol
-
 r
1.3.5.1 succinate + ubiquinone-2 succinate:quinone reductase activity is determined as quinone-mediated succinate:2,4-dichlorophenolindophenol (DCIP) reductase Plasmodium yoelii yoelii fumarate + ubiquinol
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.5.1 tetramer determined by high resolution clear-native electrophoresis and Western blot analysis. Enzyme consists of four subunits: a flavoprotein subunit (Fp, SDH1) and an iron-sulphur subunit (Ip, SDH2) form a soluble heterodimer, which binds to a membrane anchor b-type cytochrome (SDH3)/CybS (SDH4) heterodimer Plasmodium yoelii yoelii

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 complex II
-
 Plasmodium yoelii yoelii
1.3.5.1 SQR
-
 Plasmodium yoelii yoelii
1.3.5.1 succinate-quinone reductase
-
 Plasmodium yoelii yoelii
1.3.5.1 succinate:ubiquinone reductase
-
 Plasmodium yoelii yoelii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.5.1 25
-
 assay at Plasmodium yoelii yoelii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.1 8
-
 assay at Plasmodium yoelii yoelii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.5.1 0.0036
-
-
 Plasmodium yoelii yoelii carboxin
1.3.5.1 0.0046
-
-
 Plasmodium yoelii yoelii atpenin A5