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Literature summary extracted from

  • Maeda, S.; Omata, T.
    Nitrite transport activity of the ABC-type cyanate transporter of the cyanobacterium Synechococcus elongatus (2009), J. Bacteriol., 191, 3265-3272.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.4 the nitrite transporting system is encoded by the four genes nrtA, -B, -C, and -D, organized in the CynABD operon genes, physical map of the cynABDS genomic region Synechococcus elongatus

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.4 additional information the NA3 mutant of strain PCC7942, constructed by deleting the nrtABCD genes from the nirA operon, is defective in the active transport of nitrate, an insertional mutant of NA3 is constructed for each of the four genes and analyzed for its nitrite transport ability, overview Synechococcus elongatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.3.2.4 Cyanate competitively inhibits nitrite transport Synechococcus elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.4 0.001
-
 nitrite about Synechococcus elongatus
7.3.2.4 0.001
-
 nitrate about Synechococcus elongatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.4 ATP + H2O + nitrate/out Synechococcus elongatus the enzyme transports both nitrate and nitrite with high affinity ADP + phosphate + nitrate/in
-
 ?
7.3.2.4 ATP + H2O + nitrate/out Synechococcus elongatus PCC 7942 the enzyme transports both nitrate and nitrite with high affinity ADP + phosphate + nitrate/in
-
 ?
7.3.2.4 ATP + H2O + nitrite/out Synechococcus elongatus the enzyme transports both nitrate and nitrite with high affinity ADP + phosphate + nitrite/in
-
 ?
7.3.2.4 ATP + H2O + nitrite/out Synechococcus elongatus PCC 7942 the enzyme transports both nitrate and nitrite with high affinity ADP + phosphate + nitrite/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.4 Synechococcus elongatus
-
 strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D
-
7.3.2.4 Synechococcus elongatus PCC 7942
-
 strain PCC7942, ABC-type bispecific nitrate/nitrite transporter is encoded by the four genes nrtA, -B, -C, and -D
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.4 ATP + H2O + nitrate/out the enzyme transports both nitrate and nitrite with high affinity Synechococcus elongatus ADP + phosphate + nitrate/in
-
 ?
7.3.2.4 ATP + H2O + nitrate/out the enzyme transports both nitrate and nitrite with high affinity Synechococcus elongatus PCC 7942 ADP + phosphate + nitrate/in
-
 ?
7.3.2.4 ATP + H2O + nitrite/out the enzyme transports both nitrate and nitrite with high affinity Synechococcus elongatus ADP + phosphate + nitrite/in
-
 ?
7.3.2.4 ATP + H2O + nitrite/out the enzyme transports both nitrate and nitrite with high affinity Synechococcus elongatus PCC 7942 ADP + phosphate + nitrite/in
-
 ?
7.3.2.4 ATP + H2O + NO2-/out
-
 Synechococcus elongatus ADP + phosphate + NO2-/in
-
 ?
7.3.2.4 ATP + H2O + NO2-/out
-
 Synechococcus elongatus PCC 7942 ADP + phosphate + NO2-/in
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.4 ABC-type nitrate/nitrite-bispecific transporter
-
 Synechococcus elongatus
7.3.2.4 ABC-type NRT
-
 Synechococcus elongatus
7.3.2.4 ATP-binding cassette-type nitrate/nitrite-bispecific transporter
-
 Synechococcus elongatus
7.3.2.4 CynABD transporter
-
 Synechococcus elongatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.3.2.4 30
-
 assay at Synechococcus elongatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3.2.4 8.2 9.6 assay at, medium pH Synechococcus elongatus

Cofactor

EC Number Cofactor Comment Organism Structure
7.3.2.4 ATP
-
 Synechococcus elongatus