EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | Fe2+ | Fprs are induced by Fe2+ depletion but not by Fe2+ excess | Pseudomonas putida | |
1.18.1.2 | Fe3+ | 0.5 microM Fe(III)-citrate and 0.5 microM Fe(III)-EDTA induces Fprs | Pseudomonas putida | |
1.18.1.2 | H2O2 | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
1.18.1.2 | menadione | 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
1.18.1.2 | paraquat | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida | |
1.18.1.2 | tert-butyl hydroperoxide | 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.1.2 | expressed in Escherichia coli | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 0.00235 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00273 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00338 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00457 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00459 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00587 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00616 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00625 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00636 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.0065 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00666 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.007 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00751 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.00866 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01177 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01238 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.0129 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01319 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01424 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01544 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.01866 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.02967 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.05625 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 0.1076 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Pseudomonas putida | - |
wild type and mutants | - |
1.18.1.2 | Pseudomonas putida KT 2240 | - |
wild type and mutants | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.1.2 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 1.05 | - |
electron donor NADH, cofactor FAD | Pseudomonas putida |
1.18.1.2 | 2.58 | - |
electron donor NADPH, cofactor FAD | Pseudomonas putida |
1.18.1.2 | 5.47 | - |
electron donor NADH, cofactor FMN | Pseudomonas putida |
1.18.1.2 | 5.73 | - |
electron donor NADPH, cofactor FMN | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | Fe(III) citrate + NADH | - |
Pseudomonas putida | Fe(II) citrate + NAD+ | - |
? | |
1.18.1.2 | Fe(III) citrate + NADH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NAD+ | - |
? | |
1.18.1.2 | Fe(III) citrate + NADPH | - |
Pseudomonas putida | Fe(II) citrate + NADP+ | - |
? | |
1.18.1.2 | Fe(III) citrate + NADPH | - |
Pseudomonas putida KT 2240 | Fe(II) citrate + NADP+ | - |
? | |
1.18.1.2 | Fe(III)-EDTA + NADH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NAD+ | - |
? | |
1.18.1.2 | Fe(III)-EDTA + NADH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NAD+ | - |
? | |
1.18.1.2 | Fe(III)-EDTA + NADPH | - |
Pseudomonas putida | reduced Fe(III)-EDTA + NADP+ | - |
? | |
1.18.1.2 | Fe(III)-EDTA + NADPH | - |
Pseudomonas putida KT 2240 | reduced Fe(III)-EDTA + NADP+ | - |
? | |
1.18.1.2 | NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida | NAD+ + reduced FAD | - |
? | |
1.18.1.2 | NADH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH | Pseudomonas putida KT 2240 | NAD+ + reduced FAD | - |
? | |
1.18.1.2 | NADPH + FAD | assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH | Pseudomonas putida | NADP+ + reduced FAD | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin-NADP+ reductase | - |
Pseudomonas putida |
1.18.1.2 | FprA | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
1.18.1.2 | FprB | Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. | Pseudomonas putida |
1.18.1.2 | Fprs | - |
Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 1.91 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 3.11 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 5.6 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 6.18 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 6.8 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 7.13 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 7.54 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 7.71 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 8.21 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 9.18 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 10 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 10.18 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 10.28 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 10.64 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 10.77 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 11.18 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 11.92 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 13.2 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 16.44 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 17.47 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 19.24 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 20.38 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 21.72 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 37.61 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | free FAD serves as an electron carrier | Pseudomonas putida | |
1.18.1.2 | FMN | free FMN serves as an electron carrier | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.18.1.2 | metabolism | function in ferric iron metabolism | Pseudomonas putida |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 30 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 90 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 110 | - |
NADH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 550 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 720 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 770 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 840 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 930 | - |
NADH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 930 | - |
NADPH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1060 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1060 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1170 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1190 | - |
NADPH | for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1320 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1570 | - |
NADPH | for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 1680 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 2090 | - |
NADPH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 2690 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 2870 | - |
NADH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 3130 | - |
NADH | for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 3270 | - |
NADH | for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 6040 | - |
NADPH | for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 7090 | - |
NADH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida | |
1.18.1.2 | 17000 | - |
NADPH | for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor | Pseudomonas putida |