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Literature summary extracted from

  • Yeom, J.; Jeon, C.O.; Madsen, E.L.; Park, W.
    Ferredoxin-NADP+ reductase from Pseudomonas putida functions as a ferric reductase (2009), J. Bacteriol., 191, 1472-1479.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.18.1.2 Fe2+ Fprs are induced by Fe2+ depletion but not by Fe2+ excess Pseudomonas putida
1.18.1.2 Fe3+ 0.5 microM Fe(III)-citrate and 0.5 microM Fe(III)-EDTA induces Fprs Pseudomonas putida
1.18.1.2 H2O2 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB Pseudomonas putida
1.18.1.2 menadione 1.0 mM, oxidative stress induces Pseudomonas putida FprA but not FprB Pseudomonas putida
1.18.1.2 paraquat 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB Pseudomonas putida
1.18.1.2 tert-butyl hydroperoxide 0.5 mM, oxidative stress induces Pseudomonas putida FprA but not FprB Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.00235
-
NADH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00273
-
NADH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00338
-
NADH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00457
-
NADPH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.00459
-
NADPH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00587
-
NADPH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00616
-
NADH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00625
-
NADPH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.00636
-
NADPH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.0065
-
NADH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.00666
-
NADPH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.007
-
NADH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.00751
-
NADPH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.00866
-
NADH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.01177
-
NADPH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.01238
-
NADH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.0129
-
NADH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.01319
-
NADPH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 0.01424
-
NADPH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.01544
-
NADPH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.01866
-
NADH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.02967
-
NADPH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.05625
-
NADH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 0.1076
-
NADH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Pseudomonas putida
-
wild type and mutants
-
1.18.1.2 Pseudomonas putida KT 2240
-
wild type and mutants
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 1.05
-
electron donor NADH, cofactor FAD Pseudomonas putida
1.18.1.2 2.58
-
electron donor NADPH, cofactor FAD Pseudomonas putida
1.18.1.2 5.47
-
electron donor NADH, cofactor FMN Pseudomonas putida
1.18.1.2 5.73
-
electron donor NADPH, cofactor FMN Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 Fe(III) citrate + NADH
-
Pseudomonas putida Fe(II) citrate + NAD+
-
?
1.18.1.2 Fe(III) citrate + NADH
-
Pseudomonas putida KT 2240 Fe(II) citrate + NAD+
-
?
1.18.1.2 Fe(III) citrate + NADPH
-
Pseudomonas putida Fe(II) citrate + NADP+
-
?
1.18.1.2 Fe(III) citrate + NADPH
-
Pseudomonas putida KT 2240 Fe(II) citrate + NADP+
-
?
1.18.1.2 Fe(III)-EDTA + NADH
-
Pseudomonas putida reduced Fe(III)-EDTA + NAD+
-
?
1.18.1.2 Fe(III)-EDTA + NADH
-
Pseudomonas putida KT 2240 reduced Fe(III)-EDTA + NAD+
-
?
1.18.1.2 Fe(III)-EDTA + NADPH
-
Pseudomonas putida reduced Fe(III)-EDTA + NADP+
-
?
1.18.1.2 Fe(III)-EDTA + NADPH
-
Pseudomonas putida KT 2240 reduced Fe(III)-EDTA + NADP+
-
?
1.18.1.2 NADH + FAD assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH Pseudomonas putida NAD+ + reduced FAD
-
?
1.18.1.2 NADH + FAD assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADH Pseudomonas putida KT 2240 NAD+ + reduced FAD
-
?
1.18.1.2 NADPH + FAD assay contains glucose-6-phosphate and glucose-6-phosphate dehydrogenase to recover NADPH Pseudomonas putida NADP+ + reduced FAD
-
?

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
Pseudomonas putida
1.18.1.2 FprA Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. Pseudomonas putida
1.18.1.2 FprB Pseudomonas putida harbors two ferredoxin-NADP+ reductases FprA and FprB on its chromosome. Pseudomonas putida
1.18.1.2 Fprs
-
Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 1.91
-
NADPH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 3.11
-
NADH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 5.6
-
NADH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 6.18
-
NADPH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 6.8
-
NADPH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 7.13
-
NADPH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 7.54
-
NADPH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 7.71
-
NADH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 8.21
-
NADH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 9.18
-
NADH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 10
-
NADH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 10.18
-
NADH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 10.28
-
NADPH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 10.64
-
NADH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 10.77
-
NADPH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 11.18
-
NADPH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 11.92
-
NADPH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 13.2
-
NADH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 16.44
-
NADH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 17.47
-
NADH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 19.24
-
NADH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 20.38
-
NADPH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 21.72
-
NADPH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 37.61
-
NADPH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD free FAD serves as an electron carrier Pseudomonas putida
1.18.1.2 FMN free FMN serves as an electron carrier Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.18.1.2 metabolism function in ferric iron metabolism Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 30
-
NADH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 90
-
NADPH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 110
-
NADH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 550
-
NADH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 720
-
NADPH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 770
-
NADPH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 840
-
NADPH for FprA with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 930
-
NADH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 930
-
NADPH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 1060
-
NADH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 1060
-
NADH for FprB with FAD as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 1170
-
NADH for FprB in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 1190
-
NADPH for FprA with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 1320
-
NADPH for FprA in a flavin free assay, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 1570
-
NADPH for FprA in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 1680
-
NADPH for FprA with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 2090
-
NADPH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 2690
-
NADH for FprB with FMN as cofactor, using Fe(III)-EDTA as electron acceptor Pseudomonas putida
1.18.1.2 2870
-
NADH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 3130
-
NADH for FprB with FAD as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 3270
-
NADH for FprB in a flavin free assay, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 6040
-
NADPH for FprA with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 7090
-
NADH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida
1.18.1.2 17000
-
NADPH for FprB with FMN as cofactor, using Fe(III)-citrate as electron acceptor Pseudomonas putida