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Literature summary extracted from

  • Gescher, J.; Ismail, W.; Olgeschläger, E.; Eisenreich, W.; Wörth, J.; Fuchs, G.
    Aerobic benzoyl-coenzyme A (CoA) catabolic pathway in: conversion of ring cleavage product by 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase (2006), J. Bacteriol., 188, 2919-2927.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.77 expression in Escherichia coli as a protein tagged at its N terminus with maltose-binding protein Aromatoleum evansii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.77 additional information salt concentrations up to 500 mM KCl and EDTA concentrations up to 50 mM have no effect on enzyme activity Aromatoleum evansii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.77 0.016
-
 NADP+ pH 7.2 Aromatoleum evansii
1.2.1.77 0.025
-
 3,4-dehydroadipyl-CoA semialdehyde pH 7.2 Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.77 additional information salt concentrations up to 500 mM KCl have no effect on enzyme activity Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.77 54000
-
 2 * 54000 Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O Aromatoleum evansii enzyme of the aerobic benzoyl-coenzyme A catabolic pathway 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.77 Aromatoleum evansii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.77
-
 Aromatoleum evansii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.77 0.17
-
 extracts of cells grown aerobically on benzoate Aromatoleum evansii

Storage Stability

EC Number Storage Stability Organism
1.2.1.77 -20°C, enzyme could be stored without appreciable loss of activity for months at -20°C in the presence of 10% (v/v) glycerol Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O enzyme of the aerobic benzoyl-coenzyme A catabolic pathway Aromatoleum evansii 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde + NADP+ + H2O the molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1 Aromatoleum evansii 3,4-dehydroadipyl-CoA + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.77 dimer 2 * 54000 Aromatoleum evansii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.77 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
-
 Aromatoleum evansii
1.2.1.77 BoxD
-
 Aromatoleum evansii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.77 45
-
 NADP+ pH 7.2 Aromatoleum evansii
1.2.1.77 45
-
 3,4-dehydroadipyl-CoA semialdehyde pH 7.2 Aromatoleum evansii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.77 7.2
-
-
 Aromatoleum evansii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.77 6.2 8.8 50% of maximal activity at pH 6.2 and pH 8.8 Aromatoleum evansii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.77 NADP+ no activity (below 2%) with NAD+. The molar ratio of mol of converted substrate per mol of NADPH formed is estimated to be 1 Aromatoleum evansii