EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.39 | the oxygenase domain of iNOS with a C-terminal His6-tag is expressed in Escherichia coli | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | carbon monoxide | carbon monoxide down-regulates iNOS activity by reducing its expression level or by inhibiting its activity by converting it to an inactive P420 form, the presence of dithiothreitol, L-Arg, or H4B partially inhibits the iNOSP450 to iNOSP420 conversion, whereas the presence of both L-Arg and 5,6,7,8-tetrahydro-L-biopterin completely prevents the transition | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.39 | Rattus norvegicus | Q06518 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.39 | Ni-NTA column chromatography, gel filtration | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.39 | inducible nitric oxide synthase | - |
Rattus norvegicus |
1.14.13.39 | iNOS | - |
Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | 5,6,7,8-tetrahydro-L-biopterin | - |
Rattus norvegicus | |
1.14.13.39 | Calmodulin | - |
Rattus norvegicus | |
1.14.13.39 | NADPH | - |
Rattus norvegicus |