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Literature summary extracted from

  • Hayase, M.; Maekawa, A.; Yubisui, T.; Minami, Y.
    Properties, intracellular localization, and stage-specific expression of membrane-bound beta-glucosidase, BglM1, from Physarum polycephalum (2008), Int. J. Biochem. Cell Biol., 40, 2141-2150.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 castanospermine
-
 Physarum polycephalum
3.2.1.21 D-glucono-1,5-lactone
-
 Physarum polycephalum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.1
-
 4-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.1
-
 2-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.11
-
 2-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.19
-
 4-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.2
-
 cellohexaose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.26
-
 cellohexaose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.43
-
 gentiobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 0.52
-
 gentiobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 5.26
-
 cellobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 9.41
-
 cellobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 membrane
-
 Physarum polycephalum 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 130000
-
 x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum
3.2.1.21 230000
-
 x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Physarum polycephalum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21
-
 Physarum polycephalum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Physarum polycephalum 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Physarum polycephalum 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Physarum polycephalum 2 beta-D-glucose
-
 ?
3.2.1.21 cellohexaose + 5 H2O
-
 Physarum polycephalum 6 D-glucose
-
 ?
3.2.1.21 gentiobiose + H2O
-
 Physarum polycephalum 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 More enzyme exists in a 130000 Da and in a 230000 Da form. Kinetic data for some substrates are different for the two forms, while no difference is observed in optimum pH and temperature Physarum polycephalum
3.2.1.21 oligomer x * 130000, calculated, x * 130000, SDS-PAGE, and x * 230000, SDS-PAGE of enzyme purified in presence of Ca2+ or enzyme in crude extract Physarum polycephalum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
 both 13000 Da and 230000 Da form Physarum polycephalum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 60
-
 30% residual activity, both 13000 Da and 230000 Da form Physarum polycephalum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 1
-
 2-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 5
-
 cellohexaose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 8
-
 cellohexaose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 13
-
 2-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 13
-
 4-nitrophenyl beta-D-glucopyranoside 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 17
-
 gentiobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 20
-
 gentiobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 37
-
 cellobiose 230000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 103
-
 cellobiose 130000 Da form, pH 4.5, 37°C Physarum polycephalum
3.2.1.21 110
-
 4-nitrophenyl beta-D-glucopyranoside 130000 Da form, pH 4.5, 37°C Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.5
-
 both 13000 Da and 230000 Da form Physarum polycephalum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.21 0.03
-
 pH 4.5, 37°C, both 13000 Da and 230000 Da form Physarum polycephalum castanospermine
3.2.1.21 0.16
-
 pH 4.5, 37°C, both 13000 Da and 230000 Da form Physarum polycephalum D-glucono-1,5-lactone