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Literature summary extracted from

  • Liu, Z.L.; Moon, J.
    A novel NADPH-dependent aldehyde reductase gene from Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion (2009), Gene, 446, 1-10.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.2 protein expression of Y63 using GAL promoter of pYes2NT vector in yeast Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.2 38000
-
 net Y63 protein Saccharomyces cerevisiae
1.1.1.2 45000
-
 partially purified protein of the histidine–Y63 complex Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.2 Saccharomyces cerevisiae
-
 This UniProt-ID has been deleted; NRRL Y-12632
-
1.1.1.2 Saccharomyces cerevisiae NRRL Y-12632
-
 This UniProt-ID has been deleted; NRRL Y-12632
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.2 overexpressed Y63 protein partially purified by Ni-NTA column chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.2 0.27
-
 with 4-methoxybenzaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 0.58
-
 with 5-(hydroxymethyl)furan-2-carbaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 0.9
-
 with acetaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 0.99
-
 with trans-2-nonenaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 1.28
-
 with cinnamic aldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 1.31
-
 with benzoic aldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 3.63
-
 with octanaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 3.73
-
 with heptanaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 3.97
-
 with phenylacetaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 4.19
-
 with with furan-2-carbaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 4.99
-
 with valeraldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 6.34
-
 with butyraldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 6.41
-
 with propionaldehyde as substrate Saccharomyces cerevisiae
1.1.1.2 7.67
-
 with hexanaldehyde as substrate Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.2 4-methoxybenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae 4-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.2 4-methoxybenzaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae NRRL Y-12632 4-methoxybenzyl alcohol + NADP+
-
 ?
1.1.1.2 5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae (furan-2,5-diyl)dimethanol + NADP+
-
 ?
1.1.1.2 5-(hydroxymethyl)furan-2-carbaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae NRRL Y-12632 (furan-2,5-diyl)dimethanol + NADP+
-
 ?
1.1.1.2 acetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ethanol + NADP+
-
 ?
1.1.1.2 acetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae NRRL Y-12632 ethanol + NADP+
-
 ?
1.1.1.2 benzoic aldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 butyraldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 cinnamic aldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 furan-2-carbaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 heptanaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 hexanaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 additional information the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus Saccharomyces cerevisiae ?
-
 ?
1.1.1.2 additional information the enzyme has a conserved catalytic site at Tyr169-X-X-X-Lys173. An indispensable reduction catalytic triad at Ser131, Tyr169, and Lys173, and an approved cofactor-binding motif at Gly11-X-XGly14-X-X-Ala17 near the N-terminus Saccharomyces cerevisiae NRRL Y-12632 ?
-
 ?
1.1.1.2 octanaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 phenylacetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae 2-phenylethanol + NADP+
-
 ?
1.1.1.2 phenylacetaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae NRRL Y-12632 2-phenylethanol + NADP+
-
 ?
1.1.1.2 propionaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae propanol + NADP+
-
 ?
1.1.1.2 trans-2-nonenaldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?
1.1.1.2 valeraldehyde + NADPH + H+
-
 Saccharomyces cerevisiae ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.2 NADPH-dependent aldehyde reductase
-
 Saccharomyces cerevisiae
1.1.1.2 Y63 protein
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.2 25
-
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.2 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.2 5 9
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.2 NADPH prefered Saccharomyces cerevisiae