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Literature summary extracted from

  • Rath, J.; Gowri, V.S.; Chauhan, S.C.; Padmanabhan, P.K.; Srinivasan, N.; Madhubala, R.
    A glutathione-specific aldose reductase of Leishmania donovani and its potential implications for methylglyoxal detoxification pathway (2009), Gene, 429, 1-9.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 glutathione ALR shows a high level of specificity towards glutathione conjugates and very little specificity towards trypanothione conjugates Leishmania donovani

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.21 expressed in Escherichia coli cells Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.112
-
 methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione Leishmania donovani
1.1.1.21 0.4
-
 methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione Leishmania donovani

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 31700
-
 subunit, calculated from amino acid sequence Leishmania donovani
1.1.1.21 74000
-
 recombinant enzyme with a maltose binding tag, SDS-PAGE Leishmania donovani

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Leishmania donovani
-
 isolate 2001-S
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21 amylose resin column chromatography Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 acetol + NADPH
-
 Leishmania donovani propane-1,2 diol + NADP+
-
 ?
1.1.1.21 methylglyoxal + NADPH + H+
-
 Leishmania donovani acetol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.21 aldose reductase
-
 Leishmania donovani
1.1.1.21 ALR
-
 Leishmania donovani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 44.4
-
 methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione Leishmania donovani
1.1.1.21 78.4
-
 methylglyoxal recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADPH
-
 Leishmania donovani

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.21 Leishmania donovani calculated from amino acid sequence
-
 5.85