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Literature summary extracted from

  • Pushpamali, W.A.; De Zoysa, M.; Kang, H.S.; Oh, C.H.; Whang, I.; Kim, S.J.; Lee, J.
    Comparative study of two thioredoxin peroxidases from disk abalone (Haliotis discus discus): cloning, recombinant protein purification, characterization of antioxidant activities and expression analysis (2008), Fish Shellfish Immunol., 24, 294-307.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.24 dithiothreitol in the H2O2 reduction assay, AbTPx1 shows the ability to remove H2O2 without the presence of dithiothreitol. However, removal of H2O2 is promoted with the presence of dithiothreitol Haliotis discus discus
1.11.1.24 dithiothreitol in the H2O2 reduction assay, AbTPx2 shows the ability to remove H2O2 without the presence of dithiothreitol. However, removal of H2O2 is promoted with the presence of dithiothreitol Haliotis discus discus
1.11.1.24 additional information AbTPx1 gene expression is induced by oxidative stress Haliotis discus discus
1.11.1.24 additional information AbTPx2 gene expressions is induced by oxidative stress Haliotis discus discus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expressed in Escherichia coli BL21 (DE3) cells Haliotis discus discus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 64500
-
recombinant protein, SDS-PAGE Haliotis discus discus
1.11.1.24 70500
-
recombinant protein, SDS-PAGE Haliotis discus discus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Haliotis discus discus B1N693 disk abalone
-
1.11.1.24 Haliotis discus discus B1N694 disk abalone
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 amylose resin column chromatography Haliotis discus discus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 abductor
-
Haliotis discus discus
-
1.11.1.24 abductor muscle
-
Haliotis discus discus
-
1.11.1.24 alimentary canal
-
Haliotis discus discus
-
1.11.1.24 gill
-
Haliotis discus discus
-
1.11.1.24 mantle
-
Haliotis discus discus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 H2O2 + reduced thioredoxin
-
Haliotis discus discus H2O + oxidized thioredoxin
-
?
1.11.1.24 tert-butyl hydroperoxide + reduced thioredoxin
-
Haliotis discus discus tert-butanol + oxidized thioredoxin + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 AbTPx1 isozyme Haliotis discus discus
1.11.1.24 AbTPx2 isozyme Haliotis discus discus
1.11.1.24 peroxiredoxin
-
Haliotis discus discus
1.11.1.24 Prx
-
Haliotis discus discus
1.11.1.24 thioredoxin peroxidase
-
Haliotis discus discus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.24 37
-
-
Haliotis discus discus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.24 30 90 H2O2 reduction activity increases from 30 to 37°C and gradually decreases from 37 to 90°C Haliotis discus discus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.24 8
-
-
Haliotis discus discus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.24 4 10
-
Haliotis discus discus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.24 thioredoxin
-
Haliotis discus discus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Haliotis discus discus calculated from amino acid sequence
-
5.7
1.11.1.24 Haliotis discus discus calculated from amino acid sequence
-
6