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Literature summary extracted from

  • Ikeda, T.; Nakamura, M.; Arai, H.; Ishii, M.; Igarashi, Y.
    Ferredoxin-NADP reductase from the thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6 (2009), FEMS Microbiol. Lett., 297, 124-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli as recombinant protein Hydrogenobacter thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
additional information Fpr showed high specificity for NADPH; Km value for NADPH is <5 microMol, whereas that for NADH is above 2 mM Hydrogenobacter thermophilus
1.18.1.2 0.131
-
NADP+
-
Hydrogenobacter thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 24600
-
gel filtration Hydrogenobacter thermophilus
1.18.1.2 30000
-
SDS-PAGE Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 oxidized ferredoxin + NADPH Hydrogenobacter thermophilus Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 reduced ferredoxin + NADP+
-
r
1.18.1.2 oxidized ferredoxin + NADPH Hydrogenobacter thermophilus TK-6 / IAM 12695 Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 reduced ferredoxin + NADP+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Hydrogenobacter thermophilus
-
-
-
1.18.1.2 Hydrogenobacter thermophilus TK-6 / IAM 12695
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2
-
Hydrogenobacter thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 21.2
-
Vmax value is 28 micromol/min/mg when NADPH is used as the electron donor, Vmax value is 22 micromol/min/mg when reduced ferredoxin Fd1 is used as the electron donor and NADP+ as electron acceptor Hydrogenobacter thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Hydrogenobacter thermophilus NADP+ + reduced 2,6-dichlorophenolindophenol
-
r
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 NADP+ + reduced 2,6-dichlorophenolindophenol
-
r
1.18.1.2 oxidized ferredoxin + NADPH
-
Hydrogenobacter thermophilus reduced ferredoxin + NADP+
-
r
1.18.1.2 oxidized ferredoxin + NADPH Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 Hydrogenobacter thermophilus reduced ferredoxin + NADP+
-
r
1.18.1.2 oxidized ferredoxin + NADPH
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 reduced ferredoxin + NADP+
-
r
1.18.1.2 oxidized ferredoxin + NADPH Hydrogenobacter thermophilus expresses three ferredoxins: [4Fe-4S]-type Fd1 and Fd2, and [2Fe-2S]-type Fd3 Hydrogenobacter thermophilus TK-6 / IAM 12695 reduced ferredoxin + NADP+
-
r
1.18.1.2 reduced ferredoxin + NADP+ reaction is performed in presence of CoA Hydrogenobacter thermophilus oxidized ferredoxin + NADPH
-
r
1.18.1.2 reduced ferredoxin + NADP+ reaction is performed in presence of CoA Hydrogenobacter thermophilus TK-6 / IAM 12695 oxidized ferredoxin + NADPH
-
r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 24600, gel filtration Hydrogenobacter thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ reductase
-
Hydrogenobacter thermophilus
1.18.1.2 FNR
-
Hydrogenobacter thermophilus
1.18.1.2 FPR
-
Hydrogenobacter thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FMN protein contains flavin mononucleotide instead of FAD Hydrogenobacter thermophilus