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Literature summary extracted from

  • Okabe, M.; Minamino, T.; Imada, K.; Namba, K.; Kihara, M.
    Role of the N-terminal domain of FliI ATPase in bacterial flagellar protein export (2009), FEBS Lett., 583, 743-748.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.8 expression of N-terminally His-tagged wild-type and deletion mutant Flil enzymes, and expression of the deletion mutants in Salmonella typhimurium strain SJW1103 Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.8 additional information generation of in-frame 10-residue deletion mutations within the 100 residues of the N-terminal domain. The oligomerization and FliH-binding ability are retained and the ATPase activity is maintained in most of the deletion variants, except for DELTA6 mutant and partially for DELTA1 mutant, DELTA4 mutant shows inhibited motility compared to the wild-type enzyme, mutant DELTA2 and DELTA4, as well as DELTA35-38 show 1.3, 5.7, and 2fold increased ATPase activity, overview Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.8 membrane
-
 Salmonella enterica subsp. enterica serovar Typhimurium 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.8 ATP + H2O Salmonella enterica subsp. enterica serovar Typhimurium FliI, the ATPase involved in bacterial flagellar protein export, forms a complex with its regulator FliH in the cytoplasm and hexamerizes upon docking to the export gate composed of integral membrane proteins ADP + phosphate
-
 ?
7.4.2.8 ATP + H2O Salmonella enterica subsp. enterica serovar Typhimurium SJW1103 FliI, the ATPase involved in bacterial flagellar protein export, forms a complex with its regulator FliH in the cytoplasm and hexamerizes upon docking to the export gate composed of integral membrane proteins ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.8 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
7.4.2.8 Salmonella enterica subsp. enterica serovar Typhimurium SJW1103
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.8 ATP + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate
-
 ?
7.4.2.8 ATP + H2O FliI, the ATPase involved in bacterial flagellar protein export, forms a complex with its regulator FliH in the cytoplasm and hexamerizes upon docking to the export gate composed of integral membrane proteins Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate
-
 ?
7.4.2.8 ATP + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium SJW1103 ADP + phosphate
-
 ?
7.4.2.8 ATP + H2O FliI, the ATPase involved in bacterial flagellar protein export, forms a complex with its regulator FliH in the cytoplasm and hexamerizes upon docking to the export gate composed of integral membrane proteins Salmonella enterica subsp. enterica serovar Typhimurium SJW1103 ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
7.4.2.8 hexamer FliI forms a complex with its regulator FliH in the cytoplasm and hexamerizes upon docking to the export gate composed of integral membrane proteins Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.8 More structure analysis and comparison using crystal structure PDB code DPY Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
7.4.2.8 FliI
-
 Salmonella enterica subsp. enterica serovar Typhimurium
7.4.2.8 FliI ATPase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4.2.8 8
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium