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Literature summary extracted from

  • Leferink, N.G.; Jose, M.D.; van den Berg, W.A.; van Berkel, W.J.
    Functional assignment of Glu386 and Arg388 in the active site of L-galactono-gamma-lactone dehydrogenase (2009), FEBS Lett., 583, 3199-3203.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.2.3 for expression in Escherichia coli BL21DE3 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.3.2.3 E386A mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding Arabidopsis thaliana
1.3.2.3 E386D mutant, catalytically far less efficient than wild-type GALDH, Glu386 is involved in productive substrate binding Arabidopsis thaliana
1.3.2.3 R388A inactive mutant, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor Arabidopsis thaliana
1.3.2.3 R388K mutant, shows significant activity, Arg388 is crucial for the stabilization of the anionic form of the reduced FAD cofactor Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.2.3 0.17
-
 L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 10.2
-
 D-arabinono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 12.1
-
 L-galactono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 12.4
-
 L-gulono-1,4-lactone mutant E386A Arabidopsis thaliana
1.3.2.3 13.1
-
 L-gulono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 17.9
-
 L-galactono-1,4-lactone mutant E386A Arabidopsis thaliana
1.3.2.3 18.7
-
 L-gulono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 107
-
 D-arabinono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 113
-
 L-galactono-1,4-lactone mutant R388A Arabidopsis thaliana
1.3.2.3 123
-
 L-galactono-1,4-lactone mutant R388K Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.2.3 mitochondrial intermembrane space
-
 Arabidopsis thaliana 5758
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c Arabidopsis thaliana
-
 L-ascorbate + ferrocytochrome c + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.2.3 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.2.3 via His-tag Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.2.3 D-arabinono-1,4-lactone + ferrocytochrome c + H+
-
 Arabidopsis thaliana ?
-
 ?
1.3.2.3 L-galactono-1,4-lactone + ferricytochrome c
-
 Arabidopsis thaliana L-ascorbate + ferrocytochrome c + H+
-
 ?
1.3.2.3 L-gulono-1,4-lactone + ferricytochrome c
-
 Arabidopsis thaliana L-ascorbate + ferrocytochrome c
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.2.3 GALDH
-
 Arabidopsis thaliana
1.3.2.3 L-galactono-gamma-lactone dehydrogenase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.2.3 25
-
 enzyme assay Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.2.3 0.22
-
 L-gulono-1,4-lactone mutant E386A Arabidopsis thaliana
1.3.2.3 0.7
-
 L-galactono-1,4-lactone mutant R388A Arabidopsis thaliana
1.3.2.3 1.2
-
 D-arabinono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 1.4
-
 L-galactono-1,4-lactone mutant E386A Arabidopsis thaliana
1.3.2.3 4
-
 L-gulono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 4.7
-
 L-galactono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 10.4
-
 L-gulono-1,4-lactone mutant E386D Arabidopsis thaliana
1.3.2.3 48.5
-
 L-galactono-1,4-lactone mutant R388K Arabidopsis thaliana
1.3.2.3 51
-
 D-arabinono-1,4-lactone wild-type enzyme Arabidopsis thaliana
1.3.2.3 134
-
 L-galactono-1,4-lactone wild-type enzyme Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.2.3 8.8
-
 enzyme assay Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.2.3 FAD
-
 Arabidopsis thaliana