Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moi, J.W.; Crossman, L.C.; Spiro, S.; Richardson, D.J.
    The purification of ammonia monooxygenase from Paracoccus denitrificans (1996), FEBS Lett., 387, 71-74.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.39 Allylthiourea 0.5 mM, complete inhibition Paracoccus denitrificans
1.14.99.39 diethyldithiocarbamate 1 mM, complete inhibition Paracoccus denitrificans
1.14.99.39 additional information ordinary laboratory lighting inhibits ammonia monooxygenase over a period of 1 h. Acetylene is not an inhibitor at 1 mM Paracoccus denitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.99.39 membrane associated Paracoccus denitrificans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.39 copper contains a labile copper centre Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.39 38000
-
 x * 38000 + x * 46000, SDS-PAGE Paracoccus denitrificans
1.14.99.39 46000
-
 x * 38000 + x * 46000, SDS-PAGE Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.39 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.39
-
 Paracoccus denitrificans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.39 8
-
-
 Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.39 ammonia + AH2 + O2
-
 Paracoccus denitrificans NH2OH + A + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.39 ? x * 38000 + x * 46000, SDS-PAGE Paracoccus denitrificans