BRENDA - Enzyme Database

The ferredoxin-NADP+ reductase/ferredoxin electron transfer system of Plasmodium falciparum

Balconi, E.; Pennati, A.; Crobu, D.; Pandini, V.; Cerutti, R.; Zanetti, G.; Aliverti, A.; FEBS J. 276, 3825-3836 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.18.1.2
overexpressed in Escherichia coli
Plasmodium falciparum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.0008
-
Ferredoxin
Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
0.0055
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
0.01
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum
1.18.1.2
0.05
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.1.2
Plasmodium falciparum
C6KT68
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.18.1.2
-
Plasmodium falciparum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
NADPH + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
697907
Plasmodium falciparum
NADP+ + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
-
-
?
1.18.1.2
NADPH + K3Fe(CN)6
-
697907
Plasmodium falciparum
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + oxidized cytochrome c
-
697907
Plasmodium falciparum
NADP+ + reduced cytochrome c
-
-
-
r
1.18.1.2
NADPH + oxidized ferredoxin
-
697907
Plasmodium falciparum
NADP+ + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.18.1.2
More
Under oxidizing conditions, Plasmodium falciparum FNR constitutes an inactive dimeric form stabilized by an intermolecular disulfide bond. Monomer-dimer interconversion can be controlled by oxidizing and reducing agents that are possibly present within the apicoplast, such as H2O2, glutathione, and lipoate.
Plasmodium falciparum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.18.1.2
7.5
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
65
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
1.18.1.2
85
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
overexpressed in Escherichia coli
Plasmodium falciparum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.0008
-
Ferredoxin
Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
0.0055
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
0.01
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum
1.18.1.2
0.05
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.18.1.2
-
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
NADPH + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
697907
Plasmodium falciparum
NADP+ + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride
-
-
-
?
1.18.1.2
NADPH + K3Fe(CN)6
-
697907
Plasmodium falciparum
NADP+ + reduced K3Fe(CN)6
-
-
-
r
1.18.1.2
NADPH + oxidized cytochrome c
-
697907
Plasmodium falciparum
NADP+ + reduced cytochrome c
-
-
-
r
1.18.1.2
NADPH + oxidized ferredoxin
-
697907
Plasmodium falciparum
NADP+ + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.18.1.2
More
Under oxidizing conditions, Plasmodium falciparum FNR constitutes an inactive dimeric form stabilized by an intermolecular disulfide bond. Monomer-dimer interconversion can be controlled by oxidizing and reducing agents that are possibly present within the apicoplast, such as H2O2, glutathione, and lipoate.
Plasmodium falciparum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.18.1.2
7.5
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
65
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
1.18.1.2
85
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.18.1.2
1200
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
1300
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
1.18.1.2
8000
-
Ferredoxin
Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
8500
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.18.1.2
1200
-
NADPH
using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
1300
-
NADPH
using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium chloride as electron acceptor
Plasmodium falciparum
1.18.1.2
8000
-
Ferredoxin
Plasmodium falciparum ferredoxin PfFd is tested;using cytochrome c as electron acceptor
Plasmodium falciparum
1.18.1.2
8500
-
NADPH
using K3Fe(CN)6 as electron acceptor
Plasmodium falciparum