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Literature summary extracted from

  • Kotzia, G.A.; Labrou, N.E.
    Engineering thermal stability of L-asparaginase by in vitro directed evolution (2009), FEBS J., 276, 1750-1761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.1
-
 Pectobacterium carotovorum
3.5.1.1
-
 Dickeya chrysanthemi

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.1 D133I analysis of the contribution of this position on thermostability Dickeya chrysanthemi
3.5.1.1 D133L analysis of the contribution of this position on thermostability Dickeya chrysanthemi
3.5.1.1 D133T analysis of the contribution of this position on thermostability Dickeya chrysanthemi
3.5.1.1 D133V analysis of the contribution of this position on thermostability Dickeya chrysanthemi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 0.038
-
 L-asparagine mutant D133T Dickeya chrysanthemi
3.5.1.1 0.097
-
 L-asparagine mutant D133I Dickeya chrysanthemi
3.5.1.1 0.153
-
 L-asparagine mutant D133V Dickeya chrysanthemi
3.5.1.1 0.16
-
 L-asparagine mutant D133L Dickeya chrysanthemi
3.5.1.1 0.597
-
 N-acetyl-L-asparagine mutant D133V Dickeya chrysanthemi
3.5.1.1 1.008
-
 L-glutamine mutant D133T Dickeya chrysanthemi
3.5.1.1 1.099
-
 L-glutamine mutant D133I Dickeya chrysanthemi
3.5.1.1 1.677
-
 L-glutamine mutant D133L Dickeya chrysanthemi
3.5.1.1 1.788
-
 N-acetyl-L-asparagine mutant D133L Dickeya chrysanthemi
3.5.1.1 1.999
-
 L-glutamine mutant D133V Dickeya chrysanthemi
3.5.1.1 2.096
-
 N-acetyl-L-asparagine mutant D133T Dickeya chrysanthemi
3.5.1.1 5.613
-
 N-acetyl-L-asparagine mutant D133I Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Dickeya chrysanthemi Q6Q4F3
-
-
3.5.1.1 Pectobacterium carotovorum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.1
-
 Pectobacterium carotovorum
3.5.1.1
-
 Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Pectobacterium carotovorum L-aspartic acid + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Dickeya chrysanthemi L-aspartic acid + NH3
-
 ?
3.5.1.1 L-glutamine + H2O
-
 Pectobacterium carotovorum L-glutamic acid + NH3
-
 ?
3.5.1.1 L-glutamine + H2O
-
 Dickeya chrysanthemi L-glutamic acid + NH3
-
 ?
3.5.1.1 N-acetyl-L-asparagine + H2O
-
 Dickeya chrysanthemi N-acetyl-L-aspartic acid + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.1 asparaginase
-
 Pectobacterium carotovorum
3.5.1.1 asparaginase
-
 Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 46.4
-
 wild type, half-inactivation temperature, 7.5 min Dickeya chrysanthemi
3.5.1.1 50
-
 mutant D133V, half-inactivation after 159.7 h Dickeya chrysanthemi
3.5.1.1 50
-
 wild type, half-inactivation after 2.7 h Dickeya chrysanthemi
3.5.1.1 55
-
 mutant D133V, after 7.5 min 87.3% remaining activity Dickeya chrysanthemi
3.5.1.1 55
-
 wild type, after 7.5 min 19.9% remaining activity Pectobacterium carotovorum
3.5.1.1 55
-
 wild type, after 7.5 min 37.2% remaining activity Dickeya chrysanthemi
3.5.1.1 55.8
-
 mutant D133V, half-inactivation temperature, 7.5 min Dickeya chrysanthemi