EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.401 | expressed in Escherichia coli DH5alpha cells | Sphingomonas sp. |
4.1.2.53 | expressed in a L-rhamnose-defective mutant of Escherichia coli | Azotobacter vinelandii |
4.1.2.53 | expressed in a L-rhamnose-defective mutant of Escherichia coli | Scheffersomyces stipitis |
4.2.1.90 | gene LRA3, functional expression in Escherichia coli | Sphingomonas sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.90 | additional information | introduction of genes LRA1-4 or LRA1-3, LRA5 and LAR6 compensats for the L-rhamnose-defective phenotype of an Escherichia coli mutant | Sphingomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.401 | 0.646 | - |
2-dehydro-3-deoxy-L-rhamnonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 1.27 | - |
2-dehydro-3-deoxy-L-lyxonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 1.9 | - |
2-dehydro-3-deoxy-L-mannonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
4.2.1.90 | additional information | - |
additional information | the kcat/Km value with L-rhamnonate is 26.2fold and 59.2fold higher than those with L-lyxonate and L-mannonate, respectively, mainly due to 13.6fold and 30.2fold higher Km values, respectively | Sphingomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.378 | 26500 | - |
x * 26500, SDS-PAGE | Sphingomonas sp. |
1.1.1.401 | 25700 | - |
- |
Sphingomonas sp. |
1.1.1.401 | 30000 | - |
x * 30000, SDS-PAGE | Sphingomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.378 | L-rhamnose + NAD(P)+ | Sphingomonas sp. | the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway | L-rhamnono-1,4-lactone + NAD(P)H + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NAD(P)+ | Sphingomonas sp. NBRC 101715 | the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway | L-rhamnono-1,4-lactone + NAD(P)H + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-rhamnonate + NAD+ | Sphingomonas sp. | - |
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-rhamnonate + NAD+ | Sphingomonas sp. SKA58 | - |
2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Sphingomonas sp. | alternative L-rhamnose pathway | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | Sphingomonas sp. NBRC 101715 | alternative L-rhamnose pathway | L-rhamnonate | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | Azotobacter vinelandii | - |
pyruvate + (L)-lactaldehyde | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | Scheffersomyces stipitis | - |
pyruvate + (L)-lactaldehyde | - |
? | |
4.2.1.90 | L-lyxonate | Sphingomonas sp. | best substrate, 128% activity compared to L-rhamnonate | ? + H2O | - |
? | |
4.2.1.90 | L-mannonate | Sphingomonas sp. | 89.2% activity compared to L-rhamnonate | ? + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | Sphingomonas sp. | a step in the rhamnose metabolic pathway, overview | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.378 | Sphingomonas sp. | - |
- |
- |
1.1.1.378 | Sphingomonas sp. NBRC 101715 | - |
- |
- |
1.1.1.401 | Sphingomonas sp. | Q1NEI6 | - |
- |
1.1.1.401 | Sphingomonas sp. SKA58 | Q1NEI6 | - |
- |
3.1.1.65 | Sphingomonas sp. | - |
- |
- |
4.1.2.53 | Azotobacter vinelandii | - |
- |
- |
4.1.2.53 | Scheffersomyces stipitis | - |
- |
- |
4.2.1.90 | Sphingomonas sp. | Q1NEI8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.378 | - |
Sphingomonas sp. |
1.1.1.401 | Ni-NTA column chromatography and Superdex 200 gel filtration | Sphingomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.378 | 0.62 | - |
25°C, pH not specified in the publication, cell-free extract, NADP+-dependent activity | Sphingomonas sp. |
1.1.1.378 | 31.7 | - |
25°C, pH not specified in the publication, purified enzyme, NAD+-dependent activity | Sphingomonas sp. |
1.1.1.378 | 39.8 | - |
25°C, pH not specified in the publication, purified enzyme, NADP+-dependent activity | Sphingomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.378 | L-lyxose + NADP+ | activity is 75% compared to the activity with L-rhamnose and NADP+ | Sphingomonas sp. | ? | - |
? | |
1.1.1.378 | L-lyxose + NADP+ | activity is 75% compared to the activity with L-rhamnose and NADP+ | Sphingomonas sp. NBRC 101715 | ? | - |
? | |
1.1.1.378 | L-mannose + NADP+ | activity is 8.4% compared to the activity with L-rhamnose and NADP+ | Sphingomonas sp. | ? | - |
? | |
1.1.1.378 | L-mannose + NADP+ | activity is 8.4% compared to the activity with L-rhamnose and NADP+ | Sphingomonas sp. NBRC 101715 | ? | - |
? | |
1.1.1.378 | L-rhamnose + NAD(P)+ | the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway | Sphingomonas sp. | L-rhamnono-1,4-lactone + NAD(P)H + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NAD(P)+ | the enzyme is part of an alternative pathway of L-rhamnose metabolism by which L-rhamnose is converted into pyruvate and L-lactaldehyde, through reaction steps analogous to the Entner-Doudoroff pathway | Sphingomonas sp. NBRC 101715 | L-rhamnono-1,4-lactone + NAD(P)H + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NAD+ | - |
Sphingomonas sp. | L-rhamnono-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NAD+ | - |
Sphingomonas sp. NBRC 101715 | L-rhamnono-1,4-lactone + NADH + H+ | - |
? | |
1.1.1.378 | L-rhamnose + NADP+ | - |
Sphingomonas sp. | L-rhamnono-1,4-lactone + NADPH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-lyxonate + NAD+ | - |
Sphingomonas sp. | 2,4-didehydro-3-deoxy-L-lyxonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-lyxonate + NAD+ | - |
Sphingomonas sp. SKA58 | 2,4-didehydro-3-deoxy-L-lyxonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-mannonate + NAD+ | - |
Sphingomonas sp. | 2,4-didehydro-3-deoxy-L-mannonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-mannonate + NAD+ | - |
Sphingomonas sp. SKA58 | 2,4-didehydro-3-deoxy-L-mannonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-rhamnonate + NAD+ | - |
Sphingomonas sp. | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
1.1.1.401 | 2-dehydro-3-deoxy-L-rhamnonate + NAD+ | - |
Sphingomonas sp. SKA58 | 2,4-didehydro-3-deoxy-L-rhamnonate + NADH + H+ | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | alternative L-rhamnose pathway | Sphingomonas sp. | L-rhamnonate | - |
? | |
3.1.1.65 | L-rhamnono-1,4-lactone + H2O | alternative L-rhamnose pathway | Sphingomonas sp. NBRC 101715 | L-rhamnonate | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | - |
Azotobacter vinelandii | pyruvate + (L)-lactaldehyde | - |
? | |
4.1.2.53 | 2-dehydro-3-deoxy-L-rhamnonate | - |
Scheffersomyces stipitis | pyruvate + (L)-lactaldehyde | - |
? | |
4.2.1.90 | L-lyxonate | best substrate, 128% activity compared to L-rhamnonate | Sphingomonas sp. | ? + H2O | - |
? | |
4.2.1.90 | L-mannonate | 89.2% activity compared to L-rhamnonate | Sphingomonas sp. | ? + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | - |
Sphingomonas sp. | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? | |
4.2.1.90 | L-rhamnonate | a step in the rhamnose metabolic pathway, overview | Sphingomonas sp. | 2-dehydro-3-deoxy-L-rhamnonate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.378 | ? | x * 26500, SDS-PAGE | Sphingomonas sp. |
1.1.1.401 | ? | x * 30000, SDS-PAGE | Sphingomonas sp. |
1.1.1.401 | ? | x * 25700, calculated from amino acid sequence | Sphingomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.378 | EAT09360 | - |
Sphingomonas sp. |
1.1.1.378 | LRA1 | - |
Sphingomonas sp. |
1.1.1.378 | NAD(P)+-dependent L-rhamnose-1-dehydrogenase | - |
Sphingomonas sp. |
1.1.1.378 | SpLRA1 | - |
Sphingomonas sp. |
1.1.1.401 | L-2-keto-3-deoxyrhamnonate-(4)-dehydrogenase | - |
Sphingomonas sp. |
1.1.1.401 | LKDR 4-dehydrogenase | - |
Sphingomonas sp. |
1.1.1.401 | LRA5 | - |
Sphingomonas sp. |
3.1.1.65 | L-rhamnono-gamma-lactonase | - |
Sphingomonas sp. |
3.1.1.65 | LRA2 | - |
Sphingomonas sp. |
4.1.2.53 | L-2-keto-3-deoxyrhamnonate aldolase | - |
Azotobacter vinelandii |
4.1.2.53 | L-2-keto-3-deoxyrhamnonate aldolase | - |
Scheffersomyces stipitis |
4.1.2.53 | L-KDR aldolase | - |
Azotobacter vinelandii |
4.1.2.53 | L-KDR aldolase | - |
Scheffersomyces stipitis |
4.1.2.53 | LRA4 | - |
Azotobacter vinelandii |
4.1.2.53 | LRA4 | - |
Scheffersomyces stipitis |
4.2.1.90 | LRA3 | - |
Sphingomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.401 | 0.012 | - |
2-dehydro-3-deoxy-L-mannonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 0.18 | - |
2-dehydro-3-deoxy-L-lyxonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 0.86 | - |
2-dehydro-3-deoxy-L-rhamnonate | at pH 8.0 and 25°C | Sphingomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.378 | NAD+ | dual coenzyme specificity between NAD+ and NADP+ | Sphingomonas sp. | |
1.1.1.378 | NADP+ | dual coenzyme specificity between NAD+ and NADP+ | Sphingomonas sp. | |
1.1.1.401 | NAD+ | - |
Sphingomonas sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.401 | 0.0063 | - |
2-dehydro-3-deoxy-L-mannonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 0.145 | - |
2-dehydro-3-deoxy-L-lyxonate | at pH 8.0 and 25°C | Sphingomonas sp. | |
1.1.1.401 | 1.35 | - |
2-dehydro-3-deoxy-L-rhamnonate | at pH 8.0 and 25°C | Sphingomonas sp. |