EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.39 | expression in Escherichia coli | Pyrococcus furiosus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.39 | hanging drop vapour diffusion technique at 20°C, crystal structure is determined at 2.1 A resolution by molecular replacement | Pyrococcus furiosus |
3.2.1.39 | to 2.1 A resolution, determination by molecular replacement. Structure reveals a kink of six residues at the entrance of the catalytic cleft | Pyrococcus furiosus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.39 | delI72-G75 | deletion mutant, lacking residues 72-75, hydrolyses the mixed-linkage beta-1,3-1,4-glucan lichenan at 40°C and 70°C 10times more efficiently than the wiild-type protein. At 80°C, the specific activity of the D-loop mutant is reduced significantly relative to that of the wild-type with both laminarin and lichenan | Pyrococcus furiosus |
3.2.1.39 | additional information | deletion mutant lacking resiudes 72-75 hydrolyzes the mixed-linkgae beta-(1-3)-(1-4)-glucan lichenan 10times more efficiently than wild-type | Pyrococcus furiosus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.39 | Ca2+ | one ion per monomer, crystallization data | Pyrococcus furiosus | |
3.2.1.39 | Ca2+ | contains one calcium ion for each monomer. The calcium ion is located on the opposite side with respect to the catalytic cleft | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.39 | Pyrococcus furiosus | - |
- |
- |
3.2.1.39 | Pyrococcus furiosus | O73951 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.39 | 11.1 | - |
wild-type, substrate lichenan, 40°C | Pyrococcus furiosus |
3.2.1.39 | 102.8 | - |
mutant lacking residues 72-75, substrate lichenan, 80°C | Pyrococcus furiosus |
3.2.1.39 | 115.7 | - |
mutant lacking residues 72-75, substrate lichenan, 40°C | Pyrococcus furiosus |
3.2.1.39 | 115.7 | - |
wild-type, substrate lichenan, 70°C | Pyrococcus furiosus |
3.2.1.39 | 126.8 | - |
wild-type, substrate laminarin, 40°C | Pyrococcus furiosus |
3.2.1.39 | 141.4 | - |
mutant lacking residues 72-75, substrate laminarin, 40°C | Pyrococcus furiosus |
3.2.1.39 | 185 | - |
wild-type, substrate lichenan, 80°C | Pyrococcus furiosus |
3.2.1.39 | 249 | - |
mutant lacking residues 72-75, substrate laminarin, 80°C | Pyrococcus furiosus |
3.2.1.39 | 263.6 | - |
mutant lacking residues 72-75, substrate lichenan, 70°C | Pyrococcus furiosus |
3.2.1.39 | 334.3 | - |
mutant lacking residues 72-75, substrate laminarin, 70°C | Pyrococcus furiosus |
3.2.1.39 | 659.3 | - |
wild-type, substrate laminarin, 70°C | Pyrococcus furiosus |
3.2.1.39 | 1087 | - |
wild-type, substrate laminarin, 80°C | Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.39 | laminarin + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
3.2.1.39 | lichenan + H2O | - |
Pyrococcus furiosus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.39 | PfLamA | - |
Pyrococcus furiosus |