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Literature summary extracted from

  • Mendonca, L.M.; Marana, S.R.
    The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a beta-glycosidase (2008), FEBS J., 275, 2536-2547.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21
-
 Spodoptera frugiperda

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 E190Q mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones Spodoptera frugiperda
3.2.1.21 E194A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. E190 favors the binding of the initial portion of alkyl-type aglycones Spodoptera frugiperda
3.2.1.21 K201A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units Spodoptera frugiperda
3.2.1.21 K201F mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. K201 is relevant for binding of D-glucose units Spodoptera frugiperda
3.2.1.21 M453A mutant construcuted for analysis of the relative contribution of the residue to specificity and catalysis. M453 favors the binding of the second D-glucose unit of oligosaccharidic aglycones Spodoptera frugiperda

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 cellobiose
-
 Spodoptera frugiperda
3.2.1.21 cellopentaose
-
 Spodoptera frugiperda
3.2.1.21 cellotetraose
-
 Spodoptera frugiperda
3.2.1.21 cellotriose
-
 Spodoptera frugiperda
3.2.1.21 heptyl beta-D-glucoside
-
 Spodoptera frugiperda
3.2.1.21 hexyl beta-D-glucoside
-
 Spodoptera frugiperda
3.2.1.21 methyl beta-D-glucoside
-
 Spodoptera frugiperda
3.2.1.21 nonyl beta-D-glucoside
-
 Spodoptera frugiperda
3.2.1.21 octyl beta-D-glucoside
-
 Spodoptera frugiperda
3.2.1.21 phenyl beta-D-glucoside
-
 Spodoptera frugiperda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.08
-
 4-methylumbeliferyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.38
-
 4-nitrophenyl beta-D-fucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.59
-
 4-nitrophenyl beta-D-fucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.62
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.65
-
 4-nitrophenyl beta-D-fucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.66
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.67
-
 4-nitrophenyl beta-D-fucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.71
-
 4-nitrophenyl beta-D-fucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.9
-
 4-nitrophenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.04
-
 4-nitrophenyl beta-D-xyloside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.05
-
 4-nitrophenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.2
-
 4-nitrophenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.2
-
 4-nitrophenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.26
-
 4-nitrophenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.56
-
 4-nitrophenyl beta-D-xyloside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.7
-
 4-methylumbeliferyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.1
-
 4-nitrophenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.3
-
 4-methylumbeliferyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.4
-
 4-nitrophenyl beta-D-xyloside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.6
-
 4-methylumbeliferyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.6
-
 4-nitrophenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.6
-
 4-methylumbeliferyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.9
-
 4-nitrophenyl beta-D-galactoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 3.6
-
 4-nitrophenyl beta-D-xyloside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.4
-
 4-nitrophenyl beta-D-galactoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.7
-
 4-nitrophenyl beta-D-xyloside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.9
-
 4-nitrophenyl beta-D-xyloside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.9
-
 4-nitrophenyl beta-D-xyloside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.9
-
 4-nitrophenyl beta-D-galactoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 6.2
-
 4-methylumbeliferyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 6.2
-
 4-nitrophenyl beta-D-galactoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 6.3
-
 4-methylumbeliferyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 8.2
-
 4-nitrophenyl beta-D-galactoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 9
-
 4-nitrophenyl beta-D-galactoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 9.9
-
 4-nitrophenyl beta-D-galactoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Spodoptera frugiperda O61594
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbeliferyl beta-D-glucoside + H2O
-
 Spodoptera frugiperda 4-methylumbelliferol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-fucoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucoside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xyloside + H2O
-
 Spodoptera frugiperda 4-nitrophenol + D-xylose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 0.00188
-
 4-nitrophenyl beta-D-xyloside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.0073
-
 4-nitrophenyl beta-D-xyloside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.0127
-
 4-nitrophenyl beta-D-xyloside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.02
-
 4-methylumbeliferyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.0211
-
 4-nitrophenyl beta-D-xyloside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.0238
-
 4-nitrophenyl beta-D-xyloside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.0302
-
 4-nitrophenyl beta-D-xyloside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.032
-
 4-methylumbeliferyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.058
-
 4-nitrophenyl beta-D-galactoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.072
-
 4-nitrophenyl beta-D-galactoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.089
-
 4-nitrophenyl beta-D-xyloside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.114
-
 4-methylumbeliferyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.153
-
 4-nitrophenyl beta-D-galactoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.174
-
 4-nitrophenyl beta-D-galactoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.189
-
 4-nitrophenyl beta-D-galactoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.231
-
 4-methylumbeliferyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.2674
-
 4-methylumbeliferyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.269
-
 4-nitrophenyl beta-D-galactoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.348
-
 4-nitrophenyl beta-D-fucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.56
-
 4-nitrophenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.59
-
 4-nitrophenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.62
-
 4-methylumbeliferyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.65
-
 4-nitrophenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.66
-
 4-nitrophenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.668
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.98
-
 4-nitrophenyl beta-D-fucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.32
-
 4-methylumbeliferyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.52
-
 4-nitrophenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.26
-
 4-nitrophenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.43
-
 4-nitrophenyl beta-D-fucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4
-
 4-nitrophenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.03
-
 4-nitrophenyl beta-D-galactoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.15
-
 4-nitrophenyl beta-D-fucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 7.14
-
 4-nitrophenyl beta-D-fucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 8.9
-
 4-nitrophenyl beta-D-fucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 0.04
-
 cellotriose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.07
-
 cellotriose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.09
-
 cellotriose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.13
-
 nonyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.14
-
 cellotetraose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.2
-
 cellopentaose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.2
-
 cellotetraose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.21
-
 cellotetraose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.22
-
 nonyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.25
-
 cellotetraose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.25
-
 cellopentaose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.26
-
 cellopentaose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.27
-
 cellotriose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.3
-
 cellopentaose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.41
-
 cellotriose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.42
-
 octyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.44
-
 octyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.5
-
 nonyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.54
-
 cellotetraose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.7
-
 heptyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 0.83
-
 nonyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.12
-
 heptyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.16
-
 nonyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.2
-
 cellobiose mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.3
-
 octyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.49
-
 octyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 1.57
-
 hexyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2
-
 cellopentaose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.3
-
 cellotriose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.3
-
 cellopentaose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.4
-
 cellotetraose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.52
-
 octyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.6
-
 heptyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 2.9
-
 cellobiose wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 3.3
-
 hexyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 3.6
-
 heptyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 4.2
-
 heptyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 5.1
-
 methyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 5.2
-
 cellobiose mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 5.6
-
 hexyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 5.7
-
 nonyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 5.9
-
 nonyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 8
-
 methyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 8
-
 hexyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 8.6
-
 cellotriose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 10
-
 hexyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 10
-
 methyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 10.1
-
 methyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 10.2
-
 methyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 10.3
-
 octyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 11
-
 octyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 12.1
-
 methyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 13.7
-
 cellobiose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 15.6
-
 methyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 16.2
-
 cellopentaose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 17
-
 cellobiose mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 19.6
-
 cellotetraose mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 21
-
 heptyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 21.8
-
 heptyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 30
-
 phenyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 30
-
 cellobiose mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 32
-
 hexyl beta-D-glucoside mutant E190A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 36
-
 phenyl beta-D-glucoside mutant E194A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 44.9
-
 hexyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 48
-
 phenyl beta-D-glucoside mutant K201F, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 49
-
 phenyl beta-D-glucoside wild-type, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 52
-
 phenyl beta-D-glucoside mutant M453A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 74
-
 phenyl beta-D-glucoside mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 78
-
 phenyl beta-D-glucoside mutant E190Q, 30°C, pH 6.0 Spodoptera frugiperda
3.2.1.21 81
-
 cellobiose mutant K201A, 30°C, pH 6.0 Spodoptera frugiperda