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Literature summary extracted from

  • Elias, C.G.; Pereira, F.M.; Dias, F.A.; Silva, T.L.; Lopes, A.H.; dAvila-Levy, C.M.; Branquinha, M.H.; Santos, A.L.
    Cysteine peptidases in the tomato trypanosomatid Phytomonas serpens: influence of growth conditions, similarities with cruzipain and secretion to the extracellular environment (2008), Exp. Parasitol., 120, 343-352.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.B5 dithiothreitol 2 milliM, pH 5.0, 48 h, 37°C, enzymatic activity depends on reducing agents Phytomonas serpens
3.4.22.B5 L-cysteine 2 milliM, pH 5.0, 48 h, 37°C, enzymatic activity depends on reducing agents Phytomonas serpens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.B5 cystatin 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens
3.4.22.B5 leupeptin 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens
3.4.22.B5 trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane 1 microM, 37°C, 48 h, pH 5.0 Phytomonas serpens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B5 cell surface attached to membrane domains via glycosylphophatidylinositol anchor Phytomonas serpens 9986
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.B5 38000
-
 Western blotting Phytomonas serpens
3.4.22.B5 40000
-
 Western blotting Phytomonas serpens
3.4.22.51 50000
-
 Western blotting Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B5 Phytomonas serpens
-
 isolate 9T
-
3.4.22.51 Trypanosoma cruzi
-
-
-
3.4.22.51 Trypanosoma cruzi Dm28c
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.B5 37°C, 48 h, pH 5.0 Phytomonas serpens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B5 albumin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 casein 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 gelatin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 haemoglobin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 mucin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 non-immune immunoglobulin 37°C, 48 h, pH 5.0 Phytomonas serpens ?
-
 ?
3.4.22.B5 salivary gland extract 37°C, 48 h, pH 5.0, salivary gland extract from Oncopeltus fasciatus Phytomonas serpens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B5 cysteine peptidase
-
 Phytomonas serpens
3.4.22.51 cruzipain
-
 Trypanosoma cruzi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.B5 37
-
 pH 5.0, 48 h Phytomonas serpens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.B5 5
-
 37°C Phytomonas serpens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.B5 3 9 37°C, decreased enzymatic activity at pH 3.0 or neutral-alkaline pH values, 48 h Phytomonas serpens