EC Number | Crystallization (Comment) | Organism |
---|---|---|
7.4.2.6 | OppA in the open-and closed-liganded conformation, complexed with bradykinin, the nonapeptide RPPGFSPFR, hanging drop vapour diffusion method, 0.001 ml of protein solution are mixed with 10 mg/ml ligand-free OppA, 9 mM Na-MES, pH 6.0, 9 mM NaCl and 1 mM peptide, is mixed with 0.001 ml of reservoir solution containing 0.2 M NaCl, 0.1 M Na-HEPES, pH 7.0, and 20% PEG 6000, 12 weeks at room temperature, X-ray diffraction structure determination and analysis at 1.3 and 2.5 A resolution, respectively | Lactococcus lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.6 | additional information | Lactococcus lactis | the enzyme follows a mechanism for peptide selection based on amino-acid composition rather than sequence, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
7.4.2.6 | Lactococcus lactis | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
7.4.2.6 | additional information | OppA is complexed with proline-rich endogenous peptides, MALDI tandem mass spectrometry peptide analysis, overview | Lactococcus lactis |
EC Number | Purification (Comment) | Organism |
---|---|---|
7.4.2.6 | OppA without lipid anchor is produced in the cytoplasm of Lactobacillus lactis where endogenous peptides are available for binding. These peptides bind so tightly that they remain associated with the protein throughout the purification, they can be removed from the protein only by guanidium chloride treatment generating ligand-free OppA | Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
7.4.2.6 | additional information | the enzyme follows a mechanism for peptide selection based on amino-acid composition rather than sequence, overview | Lactococcus lactis | ? | - |
? | |
7.4.2.6 | additional information | OppA binds peptides of an exceptionally wide range of lengths of 4 to 35 residues with no apparent sequence preference. A huge cavity allows binding of very long peptides, and a lack of constraints for the position of the N and C termini of the ligand is compatible with binding of peptides with varying lengths, e.g. bradykinin, RPPGFSPFR | Lactococcus lactis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
7.4.2.6 | oligomer | molecular dynamics simulations, with the protein maintaing the overall open structure and shape, and structure modelling | Lactococcus lactis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
7.4.2.6 | oligopeptide-binding protein A | - |
Lactococcus lactis |
7.4.2.6 | OppA | - |
Lactococcus lactis |
7.4.2.6 | transport receptor | - |
Lactococcus lactis |