Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, Y.; Zhang, X.; Liu, Q.; Ai, C.; Mo, H.; Zeng, J.
    Expression, purification and molecular structure modeling of thioredoxin (Trx) and thioredoxin reductase (TrxR) from Acidithiobacillus ferrooxidans (2009), Curr. Microbiol., 59, 35-41.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expressed in Escherichia coli BL21(DE3) cells Acidithiobacillus ferrooxidans

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 C142A mutant shows reduced reductase activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.1.9 C142S mutant shows reduced reductase activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.1.9 C145A mutant shows reduced reductase activity compared to the wild type enzyme Acidithiobacillus ferrooxidans
1.8.1.9 C145S mutant shows reduced reductase activity compared to the wild type enzyme Acidithiobacillus ferrooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 33000
-
 SDS-PAGE Acidithiobacillus ferrooxidans

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Acidithiobacillus ferrooxidans
-
 strain ATCC 23270
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 Ni-NTA affinity column chromatography Acidithiobacillus ferrooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.9 5.1
-
 mutant enzyme C145S, in 50 mM potassium phosphate (pH 7.0), at 20°C Acidithiobacillus ferrooxidans
1.8.1.9 5.5
-
 mutant enzyme C142S, in 50 mM potassium phosphate (pH 7.0), at 20°C Acidithiobacillus ferrooxidans
1.8.1.9 5.6
-
 mutant enzyme C145A, in 50 mM potassium phosphate (pH 7.0), at 20°C Acidithiobacillus ferrooxidans
1.8.1.9 6.4
-
 mutant enzyme C142A, in 50 mM potassium phosphate (pH 7.0), at 20°C Acidithiobacillus ferrooxidans
1.8.1.9 29.7
-
 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 20°C Acidithiobacillus ferrooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 thioredoxin disulfide + NADPH + H+
-
 Acidithiobacillus ferrooxidans thioredoxin + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 thioredoxin reductase
-
 Acidithiobacillus ferrooxidans
1.8.1.9 TrxR
-
 Acidithiobacillus ferrooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Acidithiobacillus ferrooxidans
1.8.1.9 NADPH
-
 Acidithiobacillus ferrooxidans