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Literature summary extracted from

  • Fan, T.; Zhang, Y.; Yang, L.; Yang, X.; Jiang, G.; Yu, M.; Cong, R.
    Identification and characterization of a hemocyanin-derived phenoloxidase from the crab Charybdis japonica (2009), Comp. Biochem. Physiol. B, 152, 144-149.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 1-Phenyl-2-thiourea the phenoloxidase activity of HdPO is most sensitive to 1-phenyl-2-thiourea, complete inhibition at 5 mM Charybdis japonica
1.14.18.1 ascorbic acid the phenoloxidase activity of HdPO is most sensitive to ascorbic acid, 93% inhibition at 10 mM Charybdis japonica
1.14.18.1 benzoic acid 48.8% inhibition at 10 mM Charybdis japonica
1.14.18.1 Ca2+ 43.9% inhibition at 10 mM Charybdis japonica
1.14.18.1 Citric acid 27.9% inhibition at 10 mM Charybdis japonica
1.14.18.1 Cu2+ strongly inhibited by Cu2+, 92.7% inhibition at 5 mM Charybdis japonica
1.14.18.1 cysteine the phenoloxidase activity of HdPO is most sensitive to cysteine, complete inhibition at 10 mM Charybdis japonica
1.14.18.1 diethyldithiocarbamate strongly inhibited by diethyldithiocarbamate, 90.2% inhibition at 20 mM Charybdis japonica
1.14.18.1 EDTA strongly inhibited by EDTA, complete inhibition at 10 mM Charybdis japonica
1.14.18.1 Mg2+ 29.3% inhibition at 10 mM Charybdis japonica
1.14.18.1 sodium sulfite the phenoloxidase activity of HdPO is much sensitive to sodium sulfite, 72.1% inhibition at 10 mM Charybdis japonica
1.14.18.1 Thiourea the phenoloxidase activity of HdPO is much sensitive to thiourea, 83.7% inhibition at 10 mM Charybdis japonica
1.14.18.1 Zn2+ strongly inhibited by Zn2+, 80.5% inhibition at 5 mM Charybdis japonica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 2.9
-
 L-Dopa in 20 mM Tris-HCl buffer (pH 7.1) Charybdis japonica
1.14.18.1 7.33
-
 L-tyrosine in 20 mM Tris-HCl buffer (pH 7.1) Charybdis japonica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 Cu2+ HdPO is a copper-containing metalloenzyme Charybdis japonica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 70000
-
 x * 80000 + x * 75000 + n * 70000, SDS-PAGE Charybdis japonica
1.14.18.1 75000
-
 x * 80000 + x * 75000 + n * 70000, SDS-PAGE Charybdis japonica
1.14.18.1 80000
-
 x * 80000 + x * 75000 + n * 70000, SDS-PAGE Charybdis japonica

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Charybdis japonica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 DEAE Sepharose column chromatography and Sephacryl S-100 gel filtration Charybdis japonica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 hemolymph
-
 Charybdis japonica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 L-tyrosine + L-dopa + O2
-
 Charybdis japonica L-dopa + dopaquinone + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 80000 + x * 75000 + n * 70000, SDS-PAGE Charybdis japonica

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 Hc-derived phenoloxidase
-
 Charybdis japonica
1.14.18.1 HdPO
-
 Charybdis japonica
1.14.18.1 hemocyanin-derived phenoloxidase
-
 Charybdis japonica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 40
-
-
 Charybdis japonica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 7
-
-
 Charybdis japonica