Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zolotarev, A.S.; Unnikrishnan, M.; Shmukler, B.E.; Clark, J.S.; Vandorpe, D.H.; Grigorieff, N.; Rubin, E.J.; Alper, S.L.
    Increased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis (2008), Comp. Biochem. Physiol. A, 149, 255-266.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.3 inducible expression of Rv1739c in an Escherichia coli strain EWP44 lacking CysA does not increase sulfate uptake by intact cells. Expression of Rv1739c in a Mycobacterium bovis BCG strain lacking the ABC sulfate permease subunit CysA cannot complement sulfate auxotrophy. Induction of Rv1739c expression in Escherichia coli increases bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Sulfate uptake is also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.3 additional information mutation to serine of the three cysteine residues of Rv1739c does not affect magnitude, pH-dependence, or pharmacology of sulfate uptake Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.3.2.3 carbonyl cyanide 3-chloro-phenylhydrazone
-
 Mycobacterium tuberculosis
7.3.2.3 additional information no inhibition by bicarbonate, nitrate, or phosphate, or by the anion transport inhibitor NS3623 Mycobacterium tuberculosis
7.3.2.3 N-ethylmaleimide
-
 Mycobacterium tuberculosis
7.3.2.3 selenate
-
 Mycobacterium tuberculosis
7.3.2.3 sulfite
-
 Mycobacterium tuberculosis
7.3.2.3 thiosulfate
-
 Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.3 0.004
-
 sulfate pH 6.0, recombinant enzyme Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.3 additional information Na+-independent sulfate uptake Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.3 additional information Mycobacterium tuberculosis facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter ?
-
 ?
7.3.2.3 additional information Mycobacterium tuberculosis H37Rv facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.3 Mycobacterium tuberculosis
-
 gene Rv1739c
-
7.3.2.3 Mycobacterium tuberculosis H37Rv
-
 gene Rv1739c
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.3.2.3 additional information
-
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.3 ATP + H2O + sulfate/out facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter Mycobacterium tuberculosis ADP + phosphate + sulfate/in
-
 ?
7.3.2.3 ATP + H2O + sulfate/out facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter Mycobacterium tuberculosis H37Rv ADP + phosphate + sulfate/in
-
 ?
7.3.2.3 additional information facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter Mycobacterium tuberculosis ?
-
 ?
7.3.2.3 additional information Na+-independent sulfate uptake Mycobacterium tuberculosis ?
-
 ?
7.3.2.3 additional information facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter Mycobacterium tuberculosis H37Rv ?
-
 ?
7.3.2.3 additional information Na+-independent sulfate uptake Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.3 Rv1739c
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3.2.3 6
-
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.3.2.3 5 8 no activity below pH 5.0 and above pH 8.0 Mycobacterium tuberculosis