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Literature summary extracted from

  • Asada, Y.; Endo, S.; Inoue, Y.; Mamiya, H.; Hara, A.; Kunishima, N.; Matsunaga, T.
    Biochemical and structural characterization of a short-chain dehydrogenase/reductase of Thermus thermophilus HB8: a hyperthermostable aldose-1-dehydrogenase with broad substrate specificity (2009), Chem. Biol. Interact., 178, 117-126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.121 additional information kcat and Km values are increased by the elevation of temperature, whereas the kcat/Km values are not largely changed Thermus thermophilus HB8

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.121 into vector pET-11a and overexpressed in Escherichia coli BL21 (DE3) cells Thermus thermophilus HB8

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.121 by oil-microbatch method, at 1.65 A resolution. TAD follows an ordered sequential mechanism in which the coenzyme binds first Thermus thermophilus HB8

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.121 D242N mutation results in a large decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8
1.1.1.121 F141A mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8
1.1.1.121 F249A mutation results in decrease in thermostability of TAD without dissociation into dimer or monomer Thermus thermophilus HB8

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.121 1,10-phenanthroline uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
1.1.1.121 2,3-benzofuran uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
1.1.1.121 catechol
-
 Thermus thermophilus HB8
1.1.1.121 Hexestrol uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
1.1.1.121 indazole
-
 Thermus thermophilus HB8
1.1.1.121 indole uncompetitive inhibition with respect to NAD+ and competitive inhibition with respect to D-galactose Thermus thermophilus HB8
1.1.1.121 additional information EDTA (1 mM) and p-chloromercuriphenylsulfonate and p-chloromercuribenzoate (0.1 mM) have no effect on the activity Thermus thermophilus HB8

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.121 0.012
-
 isatin
-
 Thermus thermophilus HB8
1.1.1.121 0.14
-
 1-Acenaphthenol
-
 Thermus thermophilus HB8
1.1.1.121 0.17
-
 (S)-tetralol
-
 Thermus thermophilus HB8
1.1.1.121 0.25
-
 4-chromanol
-
 Thermus thermophilus HB8
1.1.1.121 0.65
-
 (S)-indanol
-
 Thermus thermophilus HB8
1.1.1.121 1.3
-
 (R)-indanol
-
 Thermus thermophilus HB8
1.1.1.121 1.9
-
 alpha-tetralone
-
 Thermus thermophilus HB8
1.1.1.121 2
-
 phenyl-1,2-propanedione
-
 Thermus thermophilus HB8
1.1.1.121 2 3 D-galactosamine
-
 Thermus thermophilus HB8
1.1.1.121 2.5
-
 (R)-tetralol
-
 Thermus thermophilus HB8
1.1.1.121 3.7
-
 2-Cyclohexen-1-ol
-
 Thermus thermophilus HB8
1.1.1.121 5.5
-
 D-fucose
-
 Thermus thermophilus HB8
1.1.1.121 27
-
 cis-benzene dihydrodiol
-
 Thermus thermophilus HB8
1.1.1.121 33
-
 2-deoxy-D-galactose
-
 Thermus thermophilus HB8
1.1.1.121 78
-
 D-galactose
-
 Thermus thermophilus HB8
1.1.1.121 131
-
 D-talose
-
 Thermus thermophilus HB8
1.1.1.121 139
-
 D-glucosamine
-
 Thermus thermophilus HB8
1.1.1.121 162
-
 L-fucose
-
 Thermus thermophilus HB8
1.1.1.121 178
-
 6-deoxy-D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 250
-
 cellobiose
-
 Thermus thermophilus HB8
1.1.1.121 262
-
 L-arabinose
-
 Thermus thermophilus HB8
1.1.1.121 300
-
 2-deoxy-D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 580
-
 D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 781
-
 lactose
-
 Thermus thermophilus HB8
1.1.1.121 841
-
 D-xylose
-
 Thermus thermophilus HB8

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.121 27000
-
 4 * 27000, SDS-PAGE Thermus thermophilus HB8
1.1.1.121 100000
-
 gel filtration Thermus thermophilus HB8

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.121 Thermus thermophilus HB8 Q5SLC4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.121 by sonication, heating at 70°C for 10 min, ion-exchange chromatography, gel filtration and ultrafiltration Thermus thermophilus HB8

Storage Stability

EC Number Storage Stability Organism
1.1.1.121 4°C Thermus thermophilus HB8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.121 (R)-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 (R)-tetralol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 (S)-(+)-1,2,3,4-tetrahydro-1-naphthol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 (S)-(+)-1-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 (S)-indanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 (S)-tetralol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 1-acenaphthenol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 2-cyclohexen-1-ol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 2-deoxy-D-galactose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 2-deoxy-D-glucose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 4-chromanol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 6-deoxy-D-glucose
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 alpha-tetralone + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
1.1.1.121 cellobiose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 cis-benzene dihydrodiol + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 D-fucose + NAD+
-
 Thermus thermophilus HB8 D-fucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-galactosamine + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 D-galactose + NAD+
-
 Thermus thermophilus HB8 D-galactono-gamma-lactone + NADH + H+
-
 ?
1.1.1.121 D-glucosamine + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 D-glucose + NAD+
-
 Thermus thermophilus HB8 D-glucono-delta-lactone + NADH + H+
-
 ?
1.1.1.121 D-talose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 D-xylose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 glucono-delta-lactone + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
1.1.1.121 isatin + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?
1.1.1.121 L-arabinose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 L-fucose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 lactose + NAD+
-
 Thermus thermophilus HB8 ? + NADH + H+
-
 ?
1.1.1.121 additional information TAD possesses the catalytic triad Ser136, Tyr149, and Lys153. No activity with L-xylose, D-ribose and 2-deoxy-D-ribose. TAD does not oxidize ketoses (D-fructose and D-sorbose), sugar alcohols (D-sorbitol, galactitol, inositol, L-arabitol and xylitol), uronic acids (D-galacturonic acid and D-glucuronic acid), aldonic acids (L-gulonic acid and 2-keto-L-gulonic acid), aromatic alcohols (benzyl alcohol and catechol), aliphatic alcohols (1-nonanol, 1-decanol, 3-hydroxybutyric acid and 2,3-butanediol), and hydroxysteroids (androsterone, testosterone, 20alpha/beta-hydroxyprogesterone, cholic acid, cortisol and corticosterone). No significant activity ketones (4-nitroacetophenone, camphorquinone, 2,3-hexanedione and diacetyl) and aldehydes (4-nitrobenzaldehyde, pyridine-3-aldehyde, 1-nonanal and citral) Thermus thermophilus HB8 ?
-
 ?
1.1.1.121 phenyl-1,2-propanedione + NADH + H+
-
 Thermus thermophilus HB8 ? + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.121 tetramer 4 * 27000, SDS-PAGE Thermus thermophilus HB8

Synonyms

EC Number Synonyms Comment Organism
1.1.1.121 TAD
-
 Thermus thermophilus HB8

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.121 80 85
-
 Thermus thermophilus HB8

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.121 85
-
 approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C Thermus thermophilus HB8

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.121 0.075
-
 (R)-tetralol
-
 Thermus thermophilus HB8
1.1.1.121 0.1067
-
 L-fucose
-
 Thermus thermophilus HB8
1.1.1.121 0.167
-
 (R)-indanol
-
 Thermus thermophilus HB8
1.1.1.121 0.3117
-
 D-galactosamine
-
 Thermus thermophilus HB8
1.1.1.121 0.423
-
 D-xylose
-
 Thermus thermophilus HB8
1.1.1.121 0.6217
-
 D-glucosamine
-
 Thermus thermophilus HB8
1.1.1.121 3.017
-
 D-talose
-
 Thermus thermophilus HB8
1.1.1.121 3.5
-
 2-Cyclohexen-1-ol
-
 Thermus thermophilus HB8
1.1.1.121 3.85
-
 D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 3.9
-
 alpha-tetralone
-
 Thermus thermophilus HB8
1.1.1.121 4.55
-
 L-arabinose
-
 Thermus thermophilus HB8
1.1.1.121 5.767
-
 cellobiose
-
 Thermus thermophilus HB8
1.1.1.121 6.35
-
 6-deoxy-D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 6.93
-
 2-deoxy-D-glucose
-
 Thermus thermophilus HB8
1.1.1.121 7.383
-
 cis-benzene dihydrodiol
-
 Thermus thermophilus HB8
1.1.1.121 8.25
-
 D-galactose
-
 Thermus thermophilus HB8
1.1.1.121 8.73
-
 D-fucose
-
 Thermus thermophilus HB8
1.1.1.121 9.23
-
 lactose
-
 Thermus thermophilus HB8
1.1.1.121 12.8
-
 2-deoxy-D-galactose
-
 Thermus thermophilus HB8
1.1.1.121 16.83
-
 phenyl-1,2-propanedione
-
 Thermus thermophilus HB8
1.1.1.121 40.3
-
 4-chromanol
-
 Thermus thermophilus HB8
1.1.1.121 138.7
-
 (S)-indanol
-
 Thermus thermophilus HB8
1.1.1.121 161.7
-
 (S)-tetralol
-
 Thermus thermophilus HB8
1.1.1.121 433.3
-
 1-Acenaphthenol
-
 Thermus thermophilus HB8
1.1.1.121 981.7
-
 isatin
-
 Thermus thermophilus HB8

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.121 2 13
-
 Thermus thermophilus HB8

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.121 additional information TAD does not exhibit dehydrogenase activity with NADP+ as the coenzyme Thermus thermophilus HB8
1.1.1.121 NAD+
-
 Thermus thermophilus HB8

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.121 0.013
-
 Hexestrol
-
 Thermus thermophilus HB8
1.1.1.121 0.025
-
 1,10-phenanthroline
-
 Thermus thermophilus HB8
1.1.1.121 0.042
-
 2,3-benzofuran
-
 Thermus thermophilus HB8
1.1.1.121 0.48
-
 indole
-
 Thermus thermophilus HB8

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.121 0.063
-
-
 Thermus thermophilus HB8 Hexestrol
1.1.1.121 0.18
-
-
 Thermus thermophilus HB8 1,10-phenanthroline
1.1.1.121 0.2
-
-
 Thermus thermophilus HB8 2,3-benzofuran
1.1.1.121 0.48
-
-
 Thermus thermophilus HB8 indole
1.1.1.121 1.2
-
-
 Thermus thermophilus HB8 catechol
1.1.1.121 1.9
-
-
 Thermus thermophilus HB8 indazole