EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.7 | ATP | essential for luminescence reaction | Luciola cruciata | |
1.13.12.7 | ATP | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
1.13.12.7 | ATP | essential for luminescence reaction, initiates luminescence reaction | Photinus pyralis | |
1.13.12.7 | CoA | prevents rapid decay of light emission | Photinus pyralis | |
1.13.12.7 | diphosphate | stimulates light production under some conditions | Photinus pyralis | |
1.13.12.7 | molecular oxygen | essential for luminescence reaction | Photinus pyralis | |
1.13.12.7 | molecular oxygen | essential for luminescence reaction | Luciola cruciata | |
1.13.12.7 | molecular oxygen | essential for luminescence reaction | Pyrophorus plagiophthalamus | |
1.13.12.7 | Triphosphate | stimulates light production under some conditions | Photinus pyralis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.12.7 | expression in Escherichia coli | Photinus pyralis |
6.2.1.3 | expression in Escherichia coli | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.12.7 | - |
Photinus pyralis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.13.12.7 | additional information | chimeric protein with fatty acyl-CoA synthetase, EC 6.2.1.3, 4% of luminescence activity from wild-tpe luciferase activity | Photinus pyralis |
1.13.12.7 | S286N | red bioluminescence | Luciola cruciata |
6.2.1.3 | C343S | no luminescence enhancement | Agrypnus binodulus |
6.2.1.3 | C343S/G344T | no luminescence enhancement | Agrypnus binodulus |
6.2.1.3 | C343S/G344T/L345S | increase of luminescence activity to 100% | Agrypnus binodulus |
6.2.1.3 | C343S/L345S | increase of luminescence activity | Agrypnus binodulus |
6.2.1.3 | G344T | no luminescence enhancement | Agrypnus binodulus |
6.2.1.3 | G344T/L345S | increase of luminescence activity to 27% | Agrypnus binodulus |
6.2.1.3 | L216R | no luminescence enhancement | Agrypnus binodulus |
6.2.1.3 | L345S | enhancement of luminescence | Agrypnus binodulus |
6.2.1.3 | additional information | chimeric protein with firefly luciferase, EC 1.13.12.7, 4% of luminescence activity from Photinus pyralis luciferase activity | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.7 | 2-phenylbenzothiazole | - |
Photinus pyralis | |
1.13.12.7 | 5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | competitive inhibitor | Photinus pyralis | |
1.13.12.7 | AMP | - |
Photinus pyralis | |
1.13.12.7 | arachidonic acid | concentration above 10 microM | Photinus pyralis | |
1.13.12.7 | ATP | - |
Photinus pyralis | |
1.13.12.7 | bromoform | - |
Photinus pyralis | |
1.13.12.7 | dehydroluciferin | - |
Luciola cruciata | |
1.13.12.7 | dehydroluciferin | - |
Photinus pyralis | |
1.13.12.7 | dehydroluciferyl adenylate | - |
Luciola cruciata | |
1.13.12.7 | dehydroluciferyl adenylate | - |
Photinus pyralis | |
1.13.12.7 | diphosphate | depending on conditions | Photinus pyralis | |
1.13.12.7 | Halothane | non-competitive inhibitor | Photinus pyralis | |
1.13.12.7 | L-luciferin | - |
Luciola cruciata | |
1.13.12.7 | L-luciferin | - |
Photinus pyralis | |
1.13.12.7 | linoleic acid | concentration above 10 microM | Photinus pyralis | |
1.13.12.7 | lipoic acid | - |
Photinus pyralis | |
1.13.12.7 | myristic acid | - |
Photinus pyralis | |
1.13.12.7 | N-pyridin-2-ylbenzamide | - |
Photinus pyralis | |
1.13.12.7 | oleic acid | concentration above 10 microM | Photinus pyralis | |
1.13.12.7 | oxyluciferin | - |
Luciola cruciata | |
1.13.12.7 | oxyluciferin | - |
Photinus pyralis | |
1.13.12.7 | pifithrin-alpha | 20 microM, above 95% inhibition | Photinus pyralis | |
6.2.1.3 | arachidonic acid | concentration above 10 microM | Drosophila melanogaster | |
6.2.1.3 | linoleic acid | concentration above 10 microM | Drosophila melanogaster | |
6.2.1.3 | oleic acid | concentration above 10 microM | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.7 | 0.0024 | - |
D-luciferin | native luciferase | Photinus pyralis | |
1.13.12.7 | 0.0024 | - |
D-luciferin | native luciferase | Luciola cruciata | |
1.13.12.7 | 0.0047 | - |
D-luciferin | recombinant luciferase | Photinus pyralis | |
1.13.12.7 | 0.0047 | - |
D-luciferin | recombinant luciferase | Luciola cruciata | |
1.13.12.7 | 0.00741 | - |
lauric acid | - |
Photinus pyralis | |
1.13.12.7 | 0.0136 | - |
linoleic acid | - |
Photinus pyralis | |
1.13.12.7 | 0.015 | - |
D-luciferin | - |
Photinus pyralis | |
1.13.12.7 | 0.0163 | - |
lauric acid | - |
Luciola cruciata | |
1.13.12.7 | 0.16 | - |
ATP | - |
Photinus pyralis | |
6.2.1.3 | 0.00168 | - |
lauric acid | - |
Drosophila melanogaster | |
6.2.1.3 | 0.00188 | - |
linoleic acid | - |
Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.7 | Mg2+ | essential | Photinus pyralis | |
1.13.12.7 | Mg2+ | essential | Luciola cruciata | |
1.13.12.7 | Mg2+ | essential | Pyrophorus plagiophthalamus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.12.7 | Aquatica lateralis | Q01158 | - |
- |
1.13.12.7 | Cratomorphus distinctus | Q5USC8 | - |
- |
1.13.12.7 | Hotaria unmunsana | Q8T6U3 | - |
- |
1.13.12.7 | Lampyris noctiluca | Q27688 | - |
- |
1.13.12.7 | Lampyris turkestanicus | Q5UFR2 | - |
- |
1.13.12.7 | Lampyroidea maculata | Q1WLP6 | - |
- |
1.13.12.7 | Luciola cruciata | P13129 | - |
- |
1.13.12.7 | Luciola mingrelica | Q26304 | - |
- |
1.13.12.7 | Luciola parvula | Q25118 | - |
- |
1.13.12.7 | Photinus pyralis | P08659 | - |
- |
1.13.12.7 | Photuris pensylvanica | Q27757 | - |
- |
1.13.12.7 | Phrixothrix hirtus | Q9U4U7 | - |
- |
1.13.12.7 | Phrixothrix vivianii | Q9U4U8 | - |
- |
1.13.12.7 | Pyrearinus termitilluminans | AF116843 | - |
- |
1.13.12.7 | Pyrocoelia miyako | Q26076 | - |
- |
1.13.12.7 | Pyrocoelia rufa | Q9GPF9 | - |
- |
1.13.12.7 | Pyrophorus mellifluus | Q717B6 | - |
- |
1.13.12.7 | Pyrophorus plagiophthalamus | Q718F0 | - |
- |
6.2.1.3 | Agrypnus binodulus | - |
- |
- |
6.2.1.3 | Drosophila melanogaster | Q9VCC6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.12.7 | ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Luciola cruciata |
1.13.12.7 | ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC | Aquatica lateralis |
1.13.12.7 | gel filtration, affinity chromatography | Photinus pyralis |
6.2.1.3 | - |
Drosophila melanogaster |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.12.7 | additional information | - |
lower luminescence activity when another substrate than D-luciferin is used: only 10% luminescence activity with 6'-amino-D-luciferin, only 5% luminescence activity with 4'-methyl-D-luciferin, only 7% luminescence activity with D-quinolylluciferin, only 1.5% luminescence activity with D-naphthylluciferin, only 16% luminescence activity with D-5,5-dimethylluciferyl-adenylate | Photinus pyralis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.12.7 | 4'-methyl-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
1.13.12.7 | 5-methylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
1.13.12.7 | 6'-amino-D-luciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
1.13.12.7 | D-5,5-dimethylluciferyl-adenylate + O2 | yellow-green color of luminescence | Photinus pyralis | ? | - |
? | |
1.13.12.7 | D-5,5-dimethylluciferyl-adenylate + O2 + ATP | red color of luminescence | Pyrophorus plagiophthalamus | ? | - |
? | |
1.13.12.7 | D-luciferin + ATP + O2 | - |
Luciola cruciata | luciferyl-adenylate + diphosphate | - |
r | |
1.13.12.7 | D-luciferin + ATP + O2 | - |
Photinus pyralis | luciferyl-adenylate + diphosphate + H2O + hv | - |
r | |
1.13.12.7 | D-naphthylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
1.13.12.7 | D-quinolylluciferin + ATP + O2 | - |
Photinus pyralis | ? | - |
? | |
1.13.12.7 | dehydroluciferin + ATP | - |
Photinus pyralis | dehydroluciferyl-AMP + diphosphate | - |
r | |
1.13.12.7 | dehydroluciferin + ATP | - |
Luciola cruciata | dehydroluciferyl-AMP + diphosphate | - |
r | |
1.13.12.7 | lauric acid + ATP + CoA | - |
Photinus pyralis | lauroyl-CoA + AMP + diphosphate | - |
? | |
1.13.12.7 | lauric acid + ATP + CoA | - |
Luciola cruciata | lauroyl-CoA + AMP + diphosphate | - |
? | |
1.13.12.7 | linoleic acid + ATP + CoA | linoleic acid concentration above 10 microM has an inhibitory effect | Photinus pyralis | linoleoyl-CoA + AMP + diphosphate | - |
? | |
1.13.12.7 | luciferyl-adenylate + O2 | - |
Photinus pyralis | oxyluciferin + AMP + CO2 + hv | - |
ir | |
1.13.12.7 | luciferyl-adenylate + O2 | - |
Luciola cruciata | oxyluciferin + AMP + CO2 + hv | - |
ir | |
1.13.12.7 | R-COOH + ATP | - |
Photinus pyralis | R-CO-AMP + diphosphate | - |
r | |
1.13.12.7 | R-COOH + ATP | - |
Luciola cruciata | R-CO-AMP + diphosphate | - |
r | |
6.2.1.3 | lauric acid | - |
Drosophila melanogaster | ? | - |
? | |
6.2.1.3 | linoleic acid + ATP + CoA | - |
Drosophila melanogaster | linoleoyl-CoA + AMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.12.7 | firefly luciferase | - |
Photinus pyralis |
1.13.12.7 | firefly luciferase | - |
Luciola cruciata |
1.13.12.7 | luciferase | - |
Cratomorphus distinctus |
1.13.12.7 | luciferase | - |
Phrixothrix vivianii |
1.13.12.7 | luciferase | - |
Phrixothrix hirtus |
1.13.12.7 | luciferase | - |
Lampyris turkestanicus |
1.13.12.7 | luciferase | - |
Luciola mingrelica |
1.13.12.7 | luciferase | - |
Pyrophorus plagiophthalamus |
1.13.12.7 | luciferase | - |
Aquatica lateralis |
1.13.12.7 | luciferase | - |
Photuris pensylvanica |
1.13.12.7 | luciferase | - |
Pyrocoelia miyako |
1.13.12.7 | luciferase | - |
Pyrocoelia rufa |
1.13.12.7 | luciferase | - |
Luciola parvula |
1.13.12.7 | luciferase | - |
Hotaria unmunsana |
1.13.12.7 | luciferase | - |
Lampyris noctiluca |
1.13.12.7 | luciferase | - |
Lampyroidea maculata |
1.13.12.7 | luciferase | - |
Pyrophorus mellifluus |
1.13.12.7 | luciferase | - |
Pyrearinus termitilluminans |
6.2.1.3 | Abll | - |
Agrypnus binodulus |
6.2.1.3 | long fatty acyl-CoA synthetase | - |
Drosophila melanogaster |
6.2.1.3 | luciferase-like gene | - |
Agrypnus binodulus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.12.7 | 5.4 | - |
red emitted light | Photinus pyralis |
1.13.12.7 | 7.6 | - |
yellow-green emitted light | Photinus pyralis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.13.12.7 | 7.5 | 8.5 | - |
Photinus pyralis |
6.2.1.3 | 7.5 | 8.5 | - |
Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.13.12.7 | ATP | - |
Photinus pyralis | |
1.13.12.7 | CoA | - |
Photinus pyralis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.12.7 | additional information | - |
dehydroluciferyl adenylate | value between 0.0000025 - 0.00125 mM | Luciola cruciata | |
1.13.12.7 | additional information | - |
5'-O-[hydroxy({[(2Z)-2-(6-oxo-1,3-benzothiazol-2(6H)-ylidene)-2,3-dihydro-1,3-thiazol-4-yl]carbonyl}oxy)phosphoryl]adenosine | value between 0.0000025-0.00125 mM | Photinus pyralis | |
1.13.12.7 | additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Photinus pyralis | |
1.13.12.7 | additional information | - |
oxyluciferin | value between 0.0005-0.002 mM | Luciola cruciata | |
1.13.12.7 | additional information | - |
L-luciferin | value between 0.003-0.004 mM | Luciola cruciata | |
1.13.12.7 | 0.000026 | - |
lipoic acid | - |
Photinus pyralis | |
1.13.12.7 | 0.000034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for D-luciferin | Photinus pyralis | |
1.13.12.7 | 0.000041 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for MgATP2- | Photinus pyralis | |
1.13.12.7 | 0.00034 | - |
5'-O-[N-(dehydroluciferyl)sulfamoyl]adenosine | for luciferyl adenylate | Photinus pyralis | |
1.13.12.7 | 0.00053 | - |
myristic acid | - |
Photinus pyralis | |
1.13.12.7 | 0.003 | 0.004 | L-luciferin | - |
Photinus pyralis |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.13.12.7 | 0.00005 | - |
- |
Photinus pyralis | lipoic acid | |
1.13.12.7 | 0.000069 | - |
- |
Photinus pyralis | N-pyridin-2-ylbenzamide | |
1.13.12.7 | 0.00068 | - |
- |
Photinus pyralis | myristic acid |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.12.7 | physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Photinus pyralis |
1.13.12.7 | physiological function | fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme | Luciola cruciata |