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Literature summary extracted from

  • Mitsuishi , M.Y.; Kobayashi , K.M.; Matsuda , M.K.
    Dextran alpha-(1 yields 2)-debranching enzyme from Flavobacterium Sp. M-73. Properties and mode of action (1980), Carbohydr. Res., 83, 303-313.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.115 Al3+ 1.33 mM, 0.9% relative activity Flavobacterium sp.
3.2.1.115 Cu2+ 1.33 mM, 1.7% relative activity Flavobacterium sp.
3.2.1.115 D-glucono-1,5-lactone strong inhibitor, 34.2% relative activity Flavobacterium sp.
3.2.1.115 Fe3+ 1.33 mM, 2.6% relative activity Flavobacterium sp.
3.2.1.115 Hg2+ 1.33 mM, 1.2% relative activity Flavobacterium sp.
3.2.1.115 iodoacetic acid still 60.1% relative activity Flavobacterium sp.
3.2.1.115 Sodium dodecyl sulfate strong inhibitor, 37.9% relative activity Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.115 0.21
-
 B-1298 soluble dextran mg/ml Flavobacterium sp.
3.2.1.115 0.27
-
 B-1299 soluble dextran mg/ml Flavobacterium sp.
3.2.1.115 0.91
-
 B-1397 soluble dextran mg/ml Flavobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.115 125000
-
 gel electrophoresis Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.115 Flavobacterium sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.115
-
 Flavobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.115 additional information
-
 5.8 micro-g glucose/ml, substrate B-1298 soluble dextran Flavobacterium sp.
3.2.1.115 additional information
-
 5.8 micro-g glucose/ml, substrate B-1299 soluble dextran Flavobacterium sp.
3.2.1.115 additional information
-
 5.8 micro-g glucose/ml, substrate B-1397 soluble dextran Flavobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.115 2(3),2(4)-di-O-alpha-glucosyl-isomaltotetraose + H2O
-
 Flavobacterium sp. isomaltotetraose + 2 alpha-D-glucose
-
 ?
3.2.1.115 2(3)-O-alpha-D-glucosyl-isomaltotetraose + H2O
-
 Flavobacterium sp. isomaltotetraose + alpha-D-glucose
-
 ?
3.2.1.115 2(3)-O-alpha-D-glucosyl-isomaltotriose + H2O
-
 Flavobacterium sp. isomaltotriose + alpha-D-glucose
-
 ?
3.2.1.115 B-1298 soluble dextran + H2O incubation at 40°C for 24 h, specifically splitz alpha-1,2-glucosidic linkages Flavobacterium sp. D-glucose + ?
-
 ?
3.2.1.115 B-1299 soluble dextran + H2O incubation at 40°C for 24 h Flavobacterium sp. D-glucose + ?
-
 ?
3.2.1.115 B-1397 soluble dextran + H2O incubation at 40°C for 24 h Flavobacterium sp. D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.115 dextran alpha-(1-->2)-debranching enzyme
-
 Flavobacterium sp.
3.2.1.115 dextranhydrolase
-
 Flavobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.115 45
-
-
 Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.115 40
-
 up to 40°C on heating for 10 min Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.115 6
-
 highest activity Flavobacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.115 6.5 9 incubation at 4°C for 24 h Flavobacterium sp.