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ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite

Hemmingsson, O.; Zhang, Y.; Still, M.; Naredi, P.; Cancer Chemother. Pharmacol. 63, 491-499 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
7.3.2.7
transfection of T-289 melanoma cells with either ASNA1 sense or ASNA1 antisense constructs, expression analysis
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
additional information
T-289 clones with siRNA decreased ASNA1 expression exhibit 51% longer doubling times than wild-type T-289 cells. After exposure to cisplatin, ASNA1 downregulated cells display a significant increase in apoptosis. The cisplatin IC50 in ASNA1 underexpressing cells is 41.7% of the IC50 for wild-type cells, and the arsenite IC50 is 59.9% of wild-type IC50. Phenotypes, overview
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Homo sapiens
-
ADP + phosphate + arsenite/out
-
-
?
7.3.2.7
ATP + H2O + cisplatin/in
Homo sapiens
-
ADP + phosphate + cisplatin/out
-
-
?
7.3.2.7
additional information
Homo sapiens
ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth.
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
7.3.2.7
Homo sapiens
O43681
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
7.3.2.7
melanoma cell
-
Homo sapiens
-
7.3.2.7
T-289 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
697072
Homo sapiens
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
ATP + H2O + cisplatin/in
-
697072
Homo sapiens
ADP + phosphate + cisplatin/out
-
-
-
?
7.3.2.7
additional information
ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth.
697072
Homo sapiens
?
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
7.3.2.7
ATP
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
7.3.2.7
transfection of T-289 melanoma cells with either ASNA1 sense or ASNA1 antisense constructs, expression analysis
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
7.3.2.7
ATP
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
7.3.2.7
additional information
T-289 clones with siRNA decreased ASNA1 expression exhibit 51% longer doubling times than wild-type T-289 cells. After exposure to cisplatin, ASNA1 downregulated cells display a significant increase in apoptosis. The cisplatin IC50 in ASNA1 underexpressing cells is 41.7% of the IC50 for wild-type cells, and the arsenite IC50 is 59.9% of wild-type IC50. Phenotypes, overview
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
Homo sapiens
-
ADP + phosphate + arsenite/out
-
-
?
7.3.2.7
ATP + H2O + cisplatin/in
Homo sapiens
-
ADP + phosphate + cisplatin/out
-
-
?
7.3.2.7
additional information
Homo sapiens
ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth.
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
7.3.2.7
melanoma cell
-
Homo sapiens
-
7.3.2.7
T-289 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7.3.2.7
ATP + H2O + arsenite/in
-
697072
Homo sapiens
ADP + phosphate + arsenite/out
-
-
-
?
7.3.2.7
ATP + H2O + cisplatin/in
-
697072
Homo sapiens
ADP + phosphate + cisplatin/out
-
-
-
?
7.3.2.7
additional information
ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth.
697072
Homo sapiens
?
-
-
-
-