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Literature summary extracted from

  • Hemmingsson, O.; Zhang, Y.; Still, M.; Naredi, P.
    ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite (2009), Cancer Chemother. Pharmacol., 63, 491-499.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.7 transfection of T-289 melanoma cells with either ASNA1 sense or ASNA1 antisense constructs, expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.7 additional information T-289 clones with siRNA decreased ASNA1 expression exhibit 51% longer doubling times than wild-type T-289 cells. After exposure to cisplatin, ASNA1 downregulated cells display a significant increase in apoptosis. The cisplatin IC50 in ASNA1 underexpressing cells is 41.7% of the IC50 for wild-type cells, and the arsenite IC50 is 59.9% of wild-type IC50. Phenotypes, overview Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in Homo sapiens
-
 ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + cisplatin/in Homo sapiens
-
 ADP + phosphate + cisplatin/out
-
 ?
7.3.2.7 additional information Homo sapiens ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth. ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.7 Homo sapiens O43681
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.3.2.7 melanoma cell
-
 Homo sapiens
-
7.3.2.7 T-289 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.7 ATP + H2O + arsenite/in
-
 Homo sapiens ADP + phosphate + arsenite/out
-
 ?
7.3.2.7 ATP + H2O + cisplatin/in
-
 Homo sapiens ADP + phosphate + cisplatin/out
-
 ?
7.3.2.7 additional information ASNA1, an ATPase targeting tail-anchored proteins, regulates melanoma cell growth and sensitivity to cisplatin and arsenite. ASNA1 is also an essential ATPase for the insertion of tail-anchored proteins into endoplasmic reticulum membranes and a regulator of insulin secretion. ASNA1 expression is necessary for growth. Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.7 ASNA1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
7.3.2.7 ATP
-
 Homo sapiens