Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Contreras-Rodriguez, A.; Quiroz-Limon, J.; Martins, A.M.; Peralta, H.; Avila-Calderon, E.; Sriranganathan, N.; Boyle, S.M.; Lopez-Merino, A.
    Enzymatic, immunological and phylogenetic characterization of Brucella suis urease (2008), BMC Microbiol., 8, 121.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 Acetohydroxamic acid
-
 Brucella suis bv. 1
3.5.1.5 Hydroxyurea
-
 Brucella suis bv. 1
3.5.1.5 Thiourea
-
 Brucella suis bv. 1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 5.6
-
 Urea by hyperbolic regression, Lineweaver-Burk: 5.24 mM Brucella suis bv. 1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 11129
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
3.5.1.5 11377
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
3.5.1.5 14000
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
3.5.1.5 15500
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
3.5.1.5 61054
-
 x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
3.5.1.5 66000
-
 x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1
3.5.1.5 130000 135000 gel filtration Brucella suis bv. 1
3.5.1.5 157000
-
 nondenaturing PAGE Brucella suis bv. 1

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Brucella suis bv. 1 Q8FZW3 urease subunit beta 2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5
-
 Brucella suis bv. 1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.5 540
-
-
 Brucella suis bv. 1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Brucella suis bv. 1 CO2 + 2 NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 ? x * 61054 + x * 11377 + x * 11129, calculated from sequence Brucella suis bv. 1
3.5.1.5 ? x * 66000 + x * 15500 + x * 14000, SDS-PAGE Brucella suis bv. 1

Synonyms

EC Number Synonyms Comment Organism
3.5.1.5 urease
-
 Brucella suis bv. 1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 28 35
-
 Brucella suis bv. 1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.5 10 45
-
 Brucella suis bv. 1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 7.3
-
-
 Brucella suis bv. 1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.5 6 8
-
 Brucella suis bv. 1

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.5 0.77
-
 Acetohydroxamic acid competitive Brucella suis bv. 1
3.5.1.5 1.04
-
 Hydroxyurea competitive Brucella suis bv. 1
3.5.1.5 26.12
-
 Thiourea competitive Brucella suis bv. 1

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.5 Brucella suis bv. 1
-
-
 5