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Literature summary extracted from

  • Heumann, S.; Eberl, A.; Fischer-Colbrie, G.; Pobeheim, H.; Kaufmann, F.; Ribitsch, D.; Cavaco-Paulo, A.; Guebitz, G.M.
    A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide (2009), Biotechnol. Bioeng., 102, 1003-1011.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.13 1-aminohexane
-
 Nocardia farcinica
3.5.1.13 AgNO3
-
 Nocardia farcinica
3.5.1.13 CuSO4
-
 Nocardia farcinica
3.5.1.13 HgCl2
-
 Nocardia farcinica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.13 0.07
-
 p-nitroacetanilide
-
 Nocardia farcinica
3.5.1.13 5.5
-
 hexanoamide
-
 Nocardia farcinica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.13 50000
-
 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica
3.5.1.13 54000
-
 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica
3.5.1.13 190000
-
 gel filtration Nocardia farcinica

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.13 Nocardia farcinica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.13
-
 Nocardia farcinica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.13 2.4
-
-
 Nocardia farcinica

Storage Stability

EC Number Storage Stability Organism
3.5.1.13 -18°C, 1 week, 18% loss of activity Nocardia farcinica
3.5.1.13 25°C, 1 week, 5% loss of activity Nocardia farcinica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.13 acetamide + H2O
-
 Nocardia farcinica acetic acid + NH3
-
 ?
3.5.1.13 adipic acid bishexylamide + H2O
-
 Nocardia farcinica adipic acid + dihexylamine
-
 ?
3.5.1.13 asparagine + H2O
-
 Nocardia farcinica aspartic acid + NH3
-
 ?
3.5.1.13 benzamide + H2O
-
 Nocardia farcinica benzoic acid + NH3
-
 ?
3.5.1.13 butyramide + H2O
-
 Nocardia farcinica butanoic acid + NH3
-
 ?
3.5.1.13 hexanoamide + ?
-
 Nocardia farcinica hexanoic acid + NH3
-
 ?
3.5.1.13 mandelamine + H2O
-
 Nocardia farcinica mandelic acid + NH3
-
 ?
3.5.1.13 methacrylamide + H2O
-
 Nocardia farcinica methacrylic acid + NH3
-
 ?
3.5.1.13 additional information surface hydrolysis of polyamide 6 Nocardia farcinica ?
-
 ?
3.5.1.13 nicotinamide + H2O
-
 Nocardia farcinica nicotinic acid + NH3
-
 ?
3.5.1.13 p-nitroacetanilide + H2O
-
 Nocardia farcinica 4-nitroaniline + acetic acid
-
 ?
3.5.1.13 propionamide + H2O
-
 Nocardia farcinica propanoic acid + NH3
-
 ?
3.5.1.13 stearamide + H2O
-
 Nocardia farcinica stearic acid + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.13 tetramer 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica

Synonyms

EC Number Synonyms Comment Organism
3.5.1.13 aryl acylamidase
-
 Nocardia farcinica
3.5.1.13 polyamidase
-
 Nocardia farcinica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.13 50
-
 pH8 Nocardia farcinica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.13 50
-
 half life time: 35 min Nocardia farcinica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.13 3.5
-
 hexanoamide
-
 Nocardia farcinica
3.5.1.13 5.8
-
 p-nitroacetanilide
-
 Nocardia farcinica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.13 8 11
-
 Nocardia farcinica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.13 6 11
-
 Nocardia farcinica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.13 0.2
-
 HgCl2
-
 Nocardia farcinica
3.5.1.13 0.2
-
 AgNO3
-
 Nocardia farcinica
3.5.1.13 2.7
-
 1-aminohexane
-
 Nocardia farcinica
3.5.1.13 3.2
-
 CuSO4
-
 Nocardia farcinica