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Literature summary extracted from

  • Inoue, Y.; Ishii, K.; Miyazaki, M.; Ueno, H.
    Purification of L-glutamate decarboxylase from monkey brain (2008), Biosci. Biotechnol. Biochem., 72, 2269-2276.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.15 L-Gln activates 18% at 1 mM Macaca fuscata

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.15 Glutarate inhibits 42% at 1 mM Macaca fuscata
4.1.1.15 L-Asn inhibits 4% at 1 mM Macaca fuscata
4.1.1.15 L-Asp inhibits 20% at 1 mM Macaca fuscata
4.1.1.15 L-Glu inhibits 25% at 1 mM Macaca fuscata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 66000
-
 gel filtration Macaca fuscata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Macaca fuscata
-
 4-aminobutanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Macaca fuscata
-
 male monkeys
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 native enzyme 550fold from brain by ammonium sulfate fractionation, hydrophobic interaction chromatography inpresence of benzamidine, ultrafiltration, anion exchange chromatography, and gel filtration Macaca fuscata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.15 brain
-
 Macaca fuscata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.15 0.0011
-
 purified native enzyme Macaca fuscata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Macaca fuscata 4-aminobutanoate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Macaca fuscata
4.1.1.15 GAD65
-
 Macaca fuscata
4.1.1.15 L-Glutamate decarboxylase
-
 Macaca fuscata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 37 50
-
 Macaca fuscata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 7 8
-
 Macaca fuscata

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
 Macaca fuscata