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Literature summary extracted from

  • Zhu, B.W.; Zhao, L.L.; Sun, L.M.; Li, D.M.; Murata, Y.; Yu, L.; Zhang, L.
    Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus (2008), Biosci. Biotechnol. Biochem., 72, 1430-1437.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.15 dithiothreitol 1 mM, 110% of initial activity Apostichopus japonicus
3.4.22.15 EDTA 1 mM, 106% of initial activity Apostichopus japonicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.15 1- (L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane i.e. E-64, 0.1 mM, complete loss of activity Apostichopus japonicus
3.4.22.15 antipain 1 mM, complete loss of activity Apostichopus japonicus
3.4.22.15 Cu2+ 1 mM, 19% residual activity Apostichopus japonicus
3.4.22.15 iodoacetic acid 1 mM, complete loss of activity Apostichopus japonicus
3.4.22.15 leupeptin 1 mM, 31% residual activity Apostichopus japonicus
3.4.22.15 Zn2+ 1 mM, complete loss of activity Apostichopus japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.15 0.069
-
 N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin pH 5.5, 37°C Apostichopus japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.15 63000
-
 x * 63000, SDS-PAGE Apostichopus japonicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.15 Apostichopus japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.15 from body wall Apostichopus japonicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.15 59.9
-
 pH 5.5, 37°C Apostichopus japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.15 L-arginine 7-amido-4-methylcoumarin + H2O very poor substrate Apostichopus japonicus ?
-
 ?
3.4.22.15 N-carbobenzoxy-L-arginine-L-arginine 7-amido-4-methylcoumarin + H2O 7% of the activity with N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin Apostichopus japonicus ?
-
 ?
3.4.22.15 N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin + H2O
-
 Apostichopus japonicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.15 ? x * 63000, SDS-PAGE Apostichopus japonicus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.15 cathepsin L-like enzyme
-
 Apostichopus japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.15 50
-
-
 Apostichopus japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.22.15 40
-
 stable below Apostichopus japonicus
3.4.22.15 55
-
 10 min, 55% residual activity Apostichopus japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.15 12.8
-
 N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin pH 5.5, 37°C Apostichopus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.15 5
-
-
 Apostichopus japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.15 5
-
 rapid decrease in activity above Apostichopus japonicus