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Literature summary extracted from

  • Hiraga, K.; Ueno, Y.; Oda, K.
    Glutamate decarboxylase from Lactobacillus brevis: activation by ammonium sulfate (2008), Biosci. Biotechnol. Biochem., 72, 1299-1306.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.15 ammonium sulfate at 1.8 M Levilactobacillus brevis
4.1.1.15 additional information sodium glutamate is essential for tetramer formation and its activation Levilactobacillus brevis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gadB, DNA and amin acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strains Rosetta-gami B (DE3) ad JM109 Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.15 1
-
 L-glutamate pH 4.6, 37°C, native enzyme Levilactobacillus brevis
4.1.1.15 1.4
-
 L-glutamate pH 4.6, 37°C, recombinant enzyme Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.15 54000
-
 2 * 54000, inactive, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
4.1.1.15 54000
-
 4 * 54000, ammonium sulfate-activated, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
4.1.1.15 110000
-
 recombinant enzyme, pH 7.0, gel filtration Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Levilactobacillus brevis
-
 4-aminobutanoate + CO2
-
 ?
4.1.1.15 L-glutamate Levilactobacillus brevis IFO 12005
-
 4-aminobutanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Levilactobacillus brevis A9ZM78 gene gadB
-
4.1.1.15 Levilactobacillus brevis IFO 12005 A9ZM78 gene gadB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.15 recombinant His-tagged enzyme from Escherichia coli strain Rosetta-gami B (DE3) by nickel affinity chromatography Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Levilactobacillus brevis 4-aminobutanoate + CO2
-
 ?
4.1.1.15 L-glutamate
-
 Levilactobacillus brevis IFO 12005 4-aminobutanoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.15 dimer 2 * 54000, inactive, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
4.1.1.15 More sodium glutamate is essential for tetramer formation and its activation Levilactobacillus brevis
4.1.1.15 tetramer 4 * 54000, ammonium sulfate-activated, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 30
-
 native enzyme Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 4.2 4.6 native enzyme Levilactobacillus brevis
4.1.1.15 4.5 5 recombinant His-tagged enzyme Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
 Levilactobacillus brevis