BRENDA - Enzyme Database show

Purification and characterization of arsenite oxidase from Arthrobacter sp.

Prasad, K.S.; Subramanian, V.; Paul, J.; Biometals 22, 711-721 (2009)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.9.1
Co2+
1 mM, 1.5% residual activity
Arthrobacter sp.
1.20.9.1
diethyldicarbonate
1 mM, 5% residual activity
Arthrobacter sp.
1.20.9.1
additional information
not inhibitory: 1 mM of Na+, Ca2+, Fe2+, Mo6+ and EDTA
Arthrobacter sp.
1.20.9.1
Zn2+
1 mM, 1.8% residual activity
Arthrobacter sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.026
-
arsenite
pH 6.0
Arthrobacter sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.9.1
membrane
about 90% of enzyme activity within the membrane fraction
Arthrobacter sp.
16020
-
1.20.9.1
periplasm
about 10% of enzyme activity within the periplasmic space
Arthrobacter sp.
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
14000
-
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
1.20.9.1
100000
-
PAGE
Arthrobacter sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.9.1
Arthrobacter sp.
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + 2,6-dichlorophenol indophenol
-
696599
Arthrobacter sp.
arsenate + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.20.9.1
dimer
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.9.1
25
-
-
Arthrobacter sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.20.9.1
5
35
more than 92% residual activity
Arthrobacter sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.20.9.1
40
-
1 h, 70% residual activity
Arthrobacter sp.
1.20.9.1
45
-
1 h, 55% residual activity
Arthrobacter sp.
1.20.9.1
50
-
1 h, 15% residual activity
Arthrobacter sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.9.1
6
-
-
Arthrobacter sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.20.9.1
5
-
78% residual activity
Arthrobacter sp.
1.20.9.1
7
-
85% residual activity
Arthrobacter sp.
1.20.9.1
8
-
82% residual activity
Arthrobacter sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.20.9.1
Co2+
1 mM, 1.5% residual activity
Arthrobacter sp.
1.20.9.1
diethyldicarbonate
1 mM, 5% residual activity
Arthrobacter sp.
1.20.9.1
additional information
not inhibitory: 1 mM of Na+, Ca2+, Fe2+, Mo6+ and EDTA
Arthrobacter sp.
1.20.9.1
Zn2+
1 mM, 1.8% residual activity
Arthrobacter sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.9.1
0.026
-
arsenite
pH 6.0
Arthrobacter sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.20.9.1
membrane
about 90% of enzyme activity within the membrane fraction
Arthrobacter sp.
16020
-
1.20.9.1
periplasm
about 10% of enzyme activity within the periplasmic space
Arthrobacter sp.
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.20.9.1
14000
-
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
1.20.9.1
100000
-
PAGE
Arthrobacter sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.9.1
arsenite + 2,6-dichlorophenol indophenol
-
696599
Arthrobacter sp.
arsenate + reduced 2,6-dichlorophenol indophenol
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.9.1
dimer
1* 85000, subunit LSU, plus 1 * 14000, subunit SSU, SDS-PAGE
Arthrobacter sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.9.1
25
-
-
Arthrobacter sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.20.9.1
5
35
more than 92% residual activity
Arthrobacter sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.20.9.1
40
-
1 h, 70% residual activity
Arthrobacter sp.
1.20.9.1
45
-
1 h, 55% residual activity
Arthrobacter sp.
1.20.9.1
50
-
1 h, 15% residual activity
Arthrobacter sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.9.1
6
-
-
Arthrobacter sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.20.9.1
5
-
78% residual activity
Arthrobacter sp.
1.20.9.1
7
-
85% residual activity
Arthrobacter sp.
1.20.9.1
8
-
82% residual activity
Arthrobacter sp.