BRENDA - Enzyme Database

Primary sequence, together with other factors, influence peptide deimination by peptidylarginine deiminase-4

Stensland, M.E.; Pollmann, S.; Molberg, ?.; Sollid, L.M.; Fleckenstein, B.; Biol. Chem. 390, 99-107 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.5.3.15
expressed in Escherichia coli BL21 cells as a fusion protein with glutathione S-transferase
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.15
0.12
-
WSRYH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.187
-
YWRDH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.27
-
MWRHV
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.325
-
KDRNW
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.345
-
WTRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.372
-
TKQTARKSTGGK
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.45
-
LDRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.575
-
TSTGGRQGSHH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.3.15
additional information
Homo sapiens
a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.5.3.15
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.5.3.15
glutathione-Sepharose 4B column chromatography
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.5.3.15
filaggrin L-Arg + H2O
-
696548
Homo sapiens
filaggrin L-citrulline + NH3
-
-
-
?
3.5.3.15
histone H3 L-arginine + H2O
-
696548
Homo sapiens
histone H3 L-citrulline + NH3
-
-
-
?
3.5.3.15
KDRNW + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
LDRGE + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
additional information
a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination
696548
Homo sapiens
?
-
-
-
-
3.5.3.15
additional information
no activity toward PVRAK, DQRTA, DVRIA, and EPRLF
696548
Homo sapiens
?
-
-
-
-
3.5.3.15
MWRHV + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
696548
Homo sapiens
N-alpha-benzoyl-L-citrulline ethyl ester
-
-
-
?
3.5.3.15
TKQTARKSTGGK + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
TSTGGRQGSHH + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
WSRYH + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
WTRGE + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
YWRDH + H2O
-
696548
Homo sapiens
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.5.3.15
PAD-4
-
Homo sapiens
3.5.3.15
peptidylarginine deiminase-4
-
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.15
0.7
-
TKQTARKSTGGK
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
1.3
-
TSTGGRQGSHH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
1.8
-
WSRYH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2
-
KDRNW
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2
-
MWRHV
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2.3
-
YWRDH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2.9
-
LDRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
3.3
-
WTRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.15
expressed in Escherichia coli BL21 cells as a fusion protein with glutathione S-transferase
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.3.15
0.12
-
WSRYH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.187
-
YWRDH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.27
-
MWRHV
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.325
-
KDRNW
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.345
-
WTRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.372
-
TKQTARKSTGGK
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.45
-
LDRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
0.575
-
TSTGGRQGSHH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.5.3.15
additional information
Homo sapiens
a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.3.15
glutathione-Sepharose 4B column chromatography
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.5.3.15
filaggrin L-Arg + H2O
-
696548
Homo sapiens
filaggrin L-citrulline + NH3
-
-
-
?
3.5.3.15
histone H3 L-arginine + H2O
-
696548
Homo sapiens
histone H3 L-citrulline + NH3
-
-
-
?
3.5.3.15
KDRNW + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
LDRGE + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
additional information
a methylated lysine residue flanking the targeted arginine influences PAD-4-mediated deimination
696548
Homo sapiens
?
-
-
-
-
3.5.3.15
additional information
no activity toward PVRAK, DQRTA, DVRIA, and EPRLF
696548
Homo sapiens
?
-
-
-
-
3.5.3.15
MWRHV + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
696548
Homo sapiens
N-alpha-benzoyl-L-citrulline ethyl ester
-
-
-
?
3.5.3.15
TKQTARKSTGGK + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
TSTGGRQGSHH + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
WSRYH + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
WTRGE + H2O
-
696548
Homo sapiens
?
-
-
-
?
3.5.3.15
YWRDH + H2O
-
696548
Homo sapiens
?
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.5.3.15
0.7
-
TKQTARKSTGGK
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
1.3
-
TSTGGRQGSHH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
1.8
-
WSRYH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2
-
KDRNW
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2
-
MWRHV
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2.3
-
YWRDH
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
2.9
-
LDRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens
3.5.3.15
3.3
-
WTRGE
in 60 mM MOPS buffer, pH 7.5 and 10 mM CaCl2, at 37C
Homo sapiens