EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.200 | amplified gene cloned into the pGEM-T Easy vector, heterologous expression with a His-tag alternatively placed in the N- or C-terminus in Escherichia coli BL21 Star (DE3) cells | Malus domestica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.200 | (NH4)2SO4 | - |
Malus domestica | |
1.1.1.200 | KCl | - |
Malus domestica | |
1.1.1.200 | additional information | no significant differences in activity when ATP, ADP, AMP, ADP-glucose, UTP, UDP-glucose, inorganic phosphate or diphosphate (5 mM each), 10% (v/v) glycerol, 500 mM glucitol or 20% (w/v) sucrose are added to the enzyme assay mixture | Malus domestica | |
1.1.1.200 | NaCl | 200 mM cause about 65% inhibition of the enzyme activity | Malus domestica | |
1.1.1.200 | NH4Cl | - |
Malus domestica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.200 | soluble | level of soluble recombinant enzyme is much higher in the N-term tagged protein than in the C-term tagged one. In the C-term tagged one the majority of the protein is in the insoluble form | Malus domestica | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.200 | 34900 | - |
sequence analysis, A6PRase | Malus domestica |
1.1.1.200 | 39000 | - |
gel filtration, C-term tagged enzyme | Malus domestica |
1.1.1.200 | 68000 | - |
gel filtration, N-term tagged enzyme | Malus domestica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.200 | Malus domestica | P28475 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.200 | one-step protein purification by immobilized metal affinity chromatography, N-term tagged protein 95% pure, C-term tagged one 75% pure | Malus domestica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.200 | leaf | - |
Malus domestica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.200 | 0.017 | - |
C-term tagged enzyme, purified enzyme | Malus domestica |
1.1.1.200 | 0.136 | - |
N-term tagged enzyme, crude extract | Malus domestica |
1.1.1.200 | 1.25 | - |
N-term tagged enzyme, 9.2fold purified | Malus domestica |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.200 | -80°C, 25 mM Tris-HCl buffer, pH 8.0, 300 mM NaCl, 1 mM beta-mercaptoethanol, 10% (v/v) glycerol, 1 mM EDTA, 0.5 mM DTT, 6 months | Malus domestica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.200 | D-glucitol-6-phosphate | - |
Malus domestica | D-glucose 6-phosphate + NADPH + H+ | - |
r | |
1.1.1.200 | D-glucose 6-phosphate + NADPH + H+ | - |
Malus domestica | D-glucitol-6-phosphate + NADP+ | - |
r | |
1.1.1.200 | additional information | both, the N- and the C-term tagged enzymes exhibit similar affinity toward substrates | Malus domestica | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.200 | dimer | gel filtration, N-term tagged enzyme | Malus domestica |
1.1.1.200 | monomer | gel filtration, C-term tagged enzyme | Malus domestica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.200 | A6PRase | - |
Malus domestica |
1.1.1.200 | aldose-6-phosphate reductase | - |
Malus domestica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.200 | 0.0011 | - |
D-glucitol-6-phosphate | C-term tagged enzyme | Malus domestica | |
1.1.1.200 | 0.009 | - |
D-glucose 6-phosphate | C-term tagged enzyme | Malus domestica | |
1.1.1.200 | 0.222 | - |
D-glucitol-6-phosphate | N-term tagged enzyme | Malus domestica | |
1.1.1.200 | 0.7 | - |
D-glucose 6-phosphate | N-term tagged enzyme | Malus domestica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.200 | 6 | 10 | N- and the C-term tagged enzymes. Both recombinant enzymes similarly show optimal activity in a broad pH range in the direction of D-glucose 6-phosphate reduction, and a narrow range (pH 9.0-10.0) in the opposite oxidative direction | Malus domestica |