EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.19 | 0.15 | - |
O2 | pH 9.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 0.15 | - |
O2 | pH 6.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 0.25 | - |
O2 | pH 7.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 0.28 | - |
O2 | pH 8.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 0.4 | - |
O2 | pH 9.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 0.45 | - |
O2 | pH 7.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 3.8 | - |
glycine | pH 8.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 5.4 | - |
glycine | pH 7.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 5.4 | - |
glycine | pH 9.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 5.6 | - |
glycine | pH 7.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 9.2 | - |
glycine | pH 6.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 14.7 | - |
glycine | pH 9.5, 25°C | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.19 | Bacillus subtilis | O31616 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.19 | glycine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + NH3 + H2O2 | free glycine oxidase forms the anionic red semiquinone upon photoreduction.This species is thermodynamically stable, as indicated by the large separation of the two single-electron reduction potentials of DeltaE 290 mV. The first potential is pH-independent, while the second is dependent. The midpoint reduction potential exhibits a 23.4 mV/pH unit slope, which is consistent with an overall two-electrons/one-proton transfer in the reduction to yield anionic reduced flavin. In the presence of glycolate and at pH 7.5 the potential for the semiquinone-reduced enzyme couple is shifted positively by about 160 mV, this favors a two-electron transfer compared to the free enzyme. Binding of glycolate and sulfite is also affected by pH | ? | |
1.4.3.19 | sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.19 | 0.9 | - |
glycine | pH 6.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 2.4 | - |
glycine | pH 7.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 3 | - |
glycine | pH 9.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 3.6 | - |
glycine | pH 8.0, 25°C | Bacillus subtilis | |
1.4.3.19 | 4 | - |
glycine | pH 8.5, 25°C | Bacillus subtilis | |
1.4.3.19 | 4.1 | - |
glycine | pH 9.0, 25°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.19 | 9 | 10 | - |
Bacillus subtilis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.4.3.19 | 6.5 | 10 | the tetrameric oligomeric state of the holoenzyme is not affected within this range | Bacillus subtilis |
1.4.3.19 | 7 | 8 | optimal range for stability | Bacillus subtilis |