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Literature summary extracted from

  • Ostrander, E.L.; Larson, J.D.; Schuermann, J.P.; Tanner, J.J.
    A conserved active site tyrosine residue of proline dehydrogenase helps enforce the preference for proline over hydroxyproline as the substrate (2009), Biochemistry, 48, 951-959.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.5.2
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.5.2 mutant Y540S Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.5.5.2 Y540A a construct consisting of residue 86-630 of PutA is used Escherichia coli
1.5.5.2 Y540S a construct consisting of residue 86-630 of PutA is used Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.5.2 L-tetrahydro-2-furoic acid
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.2 5 20 L-proline Y540A mutant Escherichia coli
1.5.5.2 59
-
 L-proline wild type Escherichia coli
1.5.5.2 100
-
 L-proline Y540S mutant Escherichia coli
1.5.5.2 190
-
 trans-4-hydroxy-L-proline Y540S mutant Escherichia coli
1.5.5.2 310
-
 trans-4-hydroxy-L-proline Y540A mutant Escherichia coli
1.5.5.2 750
-
 trans-4-hydroxy-L-proline wild type Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.2 Escherichia coli P09546
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.2
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.2 L-proline + oxidized dichlorophenolindophenol
-
 Escherichia coli 1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
 ?
1.5.5.2 trans-4-hydroxy-L-proline + oxidized dichlorophenolindophenol
-
 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.5.2 proline dehydrogenase bifunctional enzyme: proline utilization A (PutA) Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.2 2
-
 trans-4-hydroxy-L-proline Y540S mutant Escherichia coli
1.5.5.2 2.5
-
 trans-4-hydroxy-L-proline wild type Escherichia coli
1.5.5.2 2.9
-
 trans-4-hydroxy-L-proline Y540A mutant Escherichia coli
1.5.5.2 7.8
-
 L-proline Y540S mutant Escherichia coli
1.5.5.2 13
-
 L-proline Y540A mutant Escherichia coli
1.5.5.2 29
-
 L-proline wild type Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.5.2 FAD
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.5.2 0.084
-
 L-tetrahydro-2-furoic acid
-
 Escherichia coli
1.5.5.2 1.5
-
 L-tetrahydro-2-furoic acid mutant Y540S Escherichia coli
1.5.5.2 7.9
-
 L-tetrahydro-2-furoic acid mutant Y540A Escherichia coli