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Literature summary extracted from

  • Chen, N.; Zou, J.; Wang, S.; Ye, Y.; Huang, Y.; Gadda, G.; Yang, J.J.
    Designing protease sensors for real-time imaging of trypsin activation in pancreatic cancer cells (2009), Biochemistry, 48, 3519-3526.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.4 caerulein trypsinogen activation to trypsin occurs with 10 nM caerulein, trypsinogen is activated to reach a trypsin level of 6 nM following 10 nM caerulein induction for 1 h Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.4 leupeptin
-
 Homo sapiens
3.4.21.4 Soybean trypsin inhibitor
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.00399
-
 enhanced green fluorescent protein-T1 in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 0.00421
-
 enhanced green fluorescent protein-T1nb in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 0.00927
-
 tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 0.07744
-
 benzoyl-DL-Arg-7-amido-4-methylcoumarin in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 3
-
 benzoyl-DL-Arg-p-nitroanilide in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.4 enhanced green fluorescent protein-T1 + H2O Homo sapiens enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa ?
-
 ?
3.4.21.4 enhanced green fluorescent protein-T2 + H2O Homo sapiens enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa ?
-
 ?
3.4.21.4 additional information Homo sapiens does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.4 MiaPaCa-2 cell
-
 Homo sapiens
-
3.4.21.4 pancreatic cancer cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 benzoyl-DL-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzoyl-DL-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.4 benzoyl-DL-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzoyl-DL-Arg + p-nitroaniline
-
 ?
3.4.21.4 enhanced green fluorescent protein-T1 + H2O enhanced green fluorescent protein-T1 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 20 and 8 kDa Homo sapiens ?
-
 ?
3.4.21.4 enhanced green fluorescent protein-T1nb + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.4 enhanced green fluorescent protein-T2 + H2O enhanced green fluorescent protein-T2 is specifically cleaved into two major fragments by trypsin at the grafted cleavage site, approximately 18 and 10 kDa Homo sapiens ?
-
 ?
3.4.21.4 additional information does not cleave enhanced green fluorescent protein-T1m and wild type enhanced green fluorescent protein Homo sapiens ?
-
 ?
3.4.21.4 tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 0.25
-
 benzoyl-DL-Arg-7-amido-4-methylcoumarin in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 0.26
-
 enhanced green fluorescent protein-T1 in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 0.85
-
 enhanced green fluorescent protein-T1nb in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 1.33
-
 benzoyl-DL-Arg-p-nitroanilide in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens
3.4.21.4 1.41
-
 tert-butoxycarbonyl-L-Gln-L-Ala-L-Arg-7-amido-4-methylcoumarin in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 5 9 enhanced green fluorescent protein-T1 can be cleaved by trypsin under buffer conditions from pH 5 to pH 9 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.4 0.000031
-
 leupeptin in 10 mM Tris, 20 mM CaCl2, pH 7.4, at 37°C Homo sapiens