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Literature summary extracted from

  • King, M.S.; Sharpley, M.S.; Hirst, J.
    Reduction of hydrophilic ubiquinones by the flavin in mitochondrial NADH:ubiquinone oxidoreductase (Complex I) and production of reactive oxygen species (2009), Biochemistry, 48, 2053-2062.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.2 0.094
-
 NADH pH 7.6, 32°C, cosubstrate: ubiquinone-1, ubiquinone-0 or idebenone Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.1.1.2 mitochondrion NADH:ubiquinone oxidoreductase (complex I) from bovine heart mitochondria is a membrane-bound enzyme that contains 45 different subunits Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.2 Fe contains eight iron-sulfur clusters Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.1.1.2 NADH + H+ + ubiquinone-10 Bos taurus the natural substrate ubiquinone-10 is extremely hydrophobic NAD+ + ubiquinol-10
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.1.1.2 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.2 NADH + H+ + decylubiquinone
-
 Bos taurus NAD+ + decylubiquinol
-
 ?
7.1.1.2 NADH + H+ + idebenone studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ?
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-0
-
 Bos taurus NAD+ + ubiquinol-0
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-1 studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ubiquinol-1
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-10 the natural substrate ubiquinone-10 is extremely hydrophobic Bos taurus NAD+ + ubiquinol-10
-
 ?
7.1.1.2 NADH + H+ + ubiquinone-10 studies of ubiquinone reduction by isolated complex I are problematic because the extremely hydrophobic natural substrate, ubiquinone-10, must be substituted with a relatively hydrophilic analogue (such as ubiquinone-1). Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation. Moreover, semiquinones produced at the flavin site initiate redox cycling reactions with molecular oxygen, producing superoxide radicals and hydrogen peroxide. The ubiquinone reactant is regenerated, so the NADH:Q reaction becomes superstoichiometric. Idebenone, an artificial ubiquinone, reacts at the flavin site Bos taurus NAD+ + ubiquinol-10
-
 ?

Synonyms

EC Number Synonyms Comment Organism
7.1.1.2 complex I
-
 Bos taurus
7.1.1.2 NADH:ubiquinone oxidoreductase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.1.1.2 32
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.2 2.7
-
 NADH pH 7.6, 32°C, cosubstrate: ubiquinone-1, ubiquinone-0 or idebenone Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.2 7.6
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
7.1.1.2 FMN contains non-covalently bound flavin mononucleotide. Hydrophilic ubiquinones are reduced by an additional, non-energy-transducing pathway. Inhibitor-insensitive ubiquinone reduction occurs by a ping-pong type mechanism, catalyzed by the flavin mononucleotide cofactor in the active site for NADH oxidation Bos taurus
7.1.1.2 NADH
-
 Bos taurus