EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.5.2 | expression in Escherichia coli | Plasmodium falciparum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.5.2 | F188A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | F227A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | H185A | mutation decreases the affinity for Plasmodium falciparum-specific inhibitors. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | I272A | decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | L531A | decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | R265A | largest effect on Km for CoQ substrate among the mutants tested. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | Y528A | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | Y528F | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
1.3.5.2 | Y528W | substantial decrease in kcat value, decrease in FMN content. Pre-steady state kinetic analysis | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.5.2 | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | - |
Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 7.8 | - |
coenzyme Q1 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 8.8 | - |
coenzyme QD | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 11 | - |
coenzyme Q1 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 12 | - |
coenzyme QD | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 13 | - |
coenzyme QD | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 15 | - |
coenzyme Q1 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 16 | - |
coenzyme Q1 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 16 | - |
coenzyme QD | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 16 | - |
coenzyme Q1 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 17 | - |
coenzyme Q1 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 20 | - |
coenzyme QD | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 20 | - |
coenzyme QD | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 20 | - |
coenzyme Q1 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 22 | - |
coenzyme Q1 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 26 | - |
coenzyme QD | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 34 | - |
coenzyme QD | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 38 | - |
coenzyme Q1 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 45 | - |
coenzyme QD | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 48 | - |
coenzyme Q1 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 53 | - |
coenzyme QD | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.5.2 | Plasmodium falciparum | Q08210 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.5.2 | dihydroorotate + coenzyme Q1 | coenzyme Q1 possesses a prenyl tail | Plasmodium falciparum | orotate + reduced coenzyme Q1 | - |
? | |
1.3.5.2 | dihydroorotate + coenzyme QD | coenzyme QD possesses a decyl tail | Plasmodium falciparum | orotate + reduced coenzyme QD | - |
? | |
1.3.5.2 | dihydroorotate + O2 | - |
Plasmodium falciparum | orotate + H2O2 | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 0.083 | - |
O2 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.1 | - |
O2 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.12 | - |
coenzyme QD | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.18 | - |
O2 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.19 | - |
coenzyme Q1 | mutant Y528A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.25 | - |
O2 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.42 | - |
O2 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.43 | - |
O2 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.44 | - |
O2 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.53 | - |
O2 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.57 | - |
O2 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 0.63 | - |
O2 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 1.2 | - |
coenzyme QD | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 1.5 | - |
coenzyme Q1 | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 1.8 | - |
coenzyme QD | mutant I272A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 1.9 | - |
coenzyme Q1 | mutant Y528F, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 2.6 | - |
coenzyme Q1 | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 2.6 | - |
coenzyme Q1 | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 3.1 | - |
coenzyme Q1 | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 3.5 | - |
coenzyme QD | mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 4.3 | - |
coenzyme QD | mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 5 | - |
coenzyme QD | mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 5.1 | - |
coenzyme Q1 | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 5.1 | - |
coenzyme Q1 | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 6.8 | - |
coenzyme QD | mutant Y528W, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 7.3 | - |
coenzyme Q1 | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 7.8 | - |
coenzyme Q1 | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 7.9 | - |
coenzyme QD | mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 8.6 | - |
coenzyme QD | mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | |
1.3.5.2 | 12 | - |
coenzyme QD | wild-type, pH 8.0, 25°C | Plasmodium falciparum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.3.5.2 | 0.049 | - |
wild-type, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.3.5.2 | 0.087 | - |
mutant L531A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.3.5.2 | 0.1 | - |
mutant R265A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.3.5.2 | 1.9 | - |
mutant F188A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.3.5.2 | 2.9 | - |
mutant H185A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide | |
1.3.5.2 | 12 | - |
mutant F227A, pH 8.0, 25°C | Plasmodium falciparum | N-(3,5-dichloro-phenyl)-2-methyl-3-nitro-benzamide |